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Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection
Measles virus (MeV) infection of airway surface epithelial cells provides a site for final amplification before being released back into the...
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Identification of Host Factors of Paramyxoviruses by siRNA Genome-Wide Screens
Measles is a highly infectious disease that continues to spread mainly in develo** countries, often resulting in child mortality. Despite the...
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Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons
Geminiviridae is the largest and one of the most diverse families of plant viruses, comprising 14 genera demarcated based on host range, type of...
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Virus-Induced Heritable Gene Editing in Plants
Gene editing using clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) nuclease is an excellent tool for...
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Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye
Infection by positive-strand RNA viruses induces extensive remodeling of the host endomembrane system in favor of viral replication and movement. The...
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Construction of Geminivirus Infectious Clones for Agroinoculation into Plants
The production of geminiviral infectious clones provides a standardized inoculum for use in several host–virus studies. Geminiviruses present either...
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Bimolecular Fluorescence Complementation (BiFC) in Host–Virus Interactions
Bimolecular fluorescence complementation (BiFC) is an assay widely used for studying protein–protein interactions and determining the subcellular...
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Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana
More than 25% of all known plant viruses are transmitted through seeds, which makes this mode of dispersal of great importance for plant virus...
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Co-immunoprecipitation Assays to Detect Protein–Protein Interactions
Proteins usually do not function as monomers but rather perform their functions by interacting with themselves or other proteins....
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Molecular Analysis of Copy-Back Defective Interfering RNAs of Morbilliviruses
Copy-back defective interfering RNAs are major contaminants of viral stock preparations of morbilliviruses and other negative strand RNA viruses....
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Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus
We describe the use of conventional histology and immunohistochemistry against canine distemper virus (CDV) to examine the brains of domestic dogs...
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Experimental Intraperitoneal Infection of Piglets
Streptococcus suis is a swine bacterial pathogen that predominantly causes disease in weaned piglets characterized by swelling of joints, arthritis,...
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Design and Execution of In Vitro Polymerase Assays for Measles Virus and Related Mononegaviruses
Morbilliviruses such as measles virus (MeV) are responsible for major morbidity and mortality worldwide, despite the availability of an effective...
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Detection of Macrolide and/or Tetracycline Genetic Resistance Determinants by PCR
Globally, the main molecular trials being developed to study the genetic determinants responsible for conferring resistanceResistances to bacterial...
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Analysis of Susceptibility or Resistance to Antimicrobial Agents
Here were described the main three methods being used for analysis of antibiotic susceptibilitySusceptibility or resistanceResistance of...
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Measles IgG Avidity Assay
Measles IgG avidity assays determine the overall strength of molecular binding between measles-specific IgG antibodies and measles virus antigens....
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Nonviral Platform for Expression of Recombinant Protein in Insect Cells
Nonviral transfection has been used to express various recombinant proteins, therapeutics, and virus-like particles (VLP) in mammalian and insect...
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A Microsphere Immunoassay for the Quantitative Detection of Antigens in Cell Culture Supernatant
Quantitative immunoassays, such as the traditional enzyme-linked immunosorbent assay (ELISA), are used to determine concentrations of an antigen in a...
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Bacteriophage Treatment of Infected Diabetic Foot Ulcers
Diabetic foot ulcers occur as a common complication of diabetes. The concomitant infection significantly delays the healing of the ulcers. Antibiotic...
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