Search
Search Results
-
Evaluation of the Economic Impact of Streptococcus suis-Associated Disease
The economic impact of Streptococcus suis-associated disease at farm level is well known by the producers, but the cost in a region or a country is...
-
Bacterial Artificial Chromosome Reverse Genetics Approaches for SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a new member of the Coronaviridae family responsible for the coronavirus disease 19...
-
Genetic Engineering and Rebooting of Bacteriophages in L-Form Bacteria
The rapid increase of circulating, antibiotic-resistant pathogens is a major ongoing global health crisis, and arguably, the end of the “golden age...
-
Use of Galleria mellonella as an Animal Model for Studying the Antimicrobial Activity of Bacteriophages with Potential Use in Phage Therapy
Interest in phage therapy has increased in the last decade, and animal models have become essential in this field. The larval stage of the wax moth,...
-
Bacteriophage Control of Infectious Biofilms
Biofilm formation, a strategy of bacterial survival, is a significant concern in different areas, including health, where infectious biofilms are...
-
Recovery of Recombinant Rotaviruses by Reverse Genetics
Rotaviruses are the primary cause of severe gastroenteritis in infants and young children throughout the world. To combat rotavirus illness, several...
-
Plasmid-Based Lassa Virus Reverse Genetics
Several mammarenaviruses cause hemorrhagic fever (HF) disease in humans and pose a significant public health problem in their endemic regions. The...
-
A Stable Reverse Genetics System of Zika Virus Based on a Self-Splicing Group II Intron
Zika virus (ZIKV) is a mosquito-borne flavivirus of the Flaviviridae family first isolated from a sentinel monkey in the Zika Forest, Uganda, in...
-
Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase
Influenza A (FLUAV) and influenza B (FLUBV) viruses are human and/or animal pathogens widely studied due to their importance to public health and...
-
Reverse Genetics of Hepatitis C Virus Using an RNA Polymerase I-Mediated Transcription
The reverse genetics system commonly used for the production of hepatitis C virus (HCV), which is a major causative agent of liver diseases, involves...
-
Reverse Genetics Systems for the De Novo Rescue of Diverse Members of Paramyxoviridae
Paramyxoviruses place significant burdens on both human and wildlife health; while some paramyxoviruses are established within human populations,...
-
Reverse Genetics of Dengue Virus
Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering...
-
Successful Use of Phage and Antibiotics Therapy for the Eradication of Two Bacterial Pathogens from the Respiratory Tract of an Infant
Phage therapy can be a useful approach in a number of clinical cases associated with multidrug-resistant (MDR) bacterial infections. In this study,...
-
Isolation of Bacteriophages for Clinically Relevant Bacteria
The isolation of bacteriophages targeting most clinically relevant bacteria is reasonably straightforward as long as its targeted host does not have...
-
Construction of Infectious Clones for Human Enteroviruses
The infectious clone has been constructed for years via various mechanisms using reverse genetics of viral RNA into cDNA. The mechanism of...
-
Rescue of Recombinant Newcastle Disease Virus Expressing Heterologous Genes
Reverse genetics allows for the generation of recombinant infectious viruses from viral sequences or complete viral genomes cloned into plasmids....
-
Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins
Many negative-sense single-stranded RNA viruses within the order Mononegavirales harm humans. A common feature shared among cells infected by these...
-
In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells
Domestic cats are the natural host of feline morbilliviruses (FeMV). Although other species can also be infected (such as dogs and opossums), no...
-
Optical Control of Mononegavirus Gene Expression and Replication
Mononegaviruses are promising tools as oncolytic and transgene vectors for gene therapy and regenerative medicine. However, when mononegaviruses are...
-
Canine Distemper Virus Pathogenesis in the Ferret Model
Canine distemper virus (CDV) is a highly contagious pathogen within the morbillivirus genus infecting a wide range of different carnivore species....