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Phage-Host Interaction Analysis Using Flow Cytometry
Phage-host interactions are commonly evaluated by culture-based methods. However, these techniques are very laborious and time-consuming. Therefore,...
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Optimized Recovery of Viral DNA and RNA from Blood Plasma for Viral Metagenomics
Metagenomics is vastly improving our ability to discover new viruses, as well as their possible associations with disease. However, metagenomics has...
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Exploration and Retrieval of Virus-Related Molecular Data Using ExTaxsI: The Monkeypox Use Case
Retrieval and visualization of biological data are essential for understanding complex systems. With the increasing volume of data generated from...
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TurboID-Based Proximity Labeling: A Method to Decipher Protein–Protein Interactions in Plants
Proteins form complex networks through interaction to drive biological processes. Thus, dissecting protein–protein interactions (PPIs) is essential...
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Ribosome Profiling of Plants
Translation is a key step in control of gene expression, yet most analyses of global responses to a stimulus focus on transcription and the...
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Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodies
In all cases when a bacteriophage makes a direct contact with a mammalian organism, it may challenge the mammalian immunological system. Its major...
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Studying Bacteriophage Efficacy Using a Zebrafish Model
The rise of bacteria resistant to the antibiotics currently in use (multiple drug-resistant, MDR) is a serious problem for patients affected by...
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Genetic Engineering of Therapeutic Phages Using Type III CRISPR-Cas Systems
The functional characterization of “hypothetical” phage genes is a major bottleneck in basic and applied phage research. To compound this issue, the...
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Robust and Reproducible Protocol for Phage Genome “Rebooting” Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid
Production of infectious bacteriophage based on its genome is one of the necessary steps in the pipeline of editing phage genomes and creating...
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Reverse Genetics of Bat Influenza A Viruses
New World fruit bats were recently found to harbor two distinct and previously unknown influenza A viruses (IAVs) of the subtypes H17N10 and H18N11....
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Bacteriophage Taxonomy: A Continually Evolving Discipline
While taxonomy is an often underappreciated branch of science, it serves very important roles. Bacteriophage taxonomy has evolved from a discipline...
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Measles Foci Reduction Neutralization Test (FRNT)
The plaque reduction neutralization test (PRNT) and the enzyme-linked immunosorbent assay (ELISA) are both widely used to assess immunity to...
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Laboratory Methods for Culture and Identification
This chapter addresses the cultivation, identification, and characterization of Streptococcus suis. Here, we describe in detail the most used...
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Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics
RNA viruses generate defective genomes naturally during virus replication. Defective genomes that interfere with the infection dynamics either...
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Multiplex Bead Assay for the Serological Surveillance of Measles and Rubella
There is increasing interest in evaluating antibody responses to multiple antigen targets in a single assay. Immunity to measles and rubella are...
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Detection and Quantification of Conjugative Transfer of Mobile Genetic Elements Carrying Antibiotic Resistance Genes
Multidrug resistance, due to acquired antimicrobial resistance genes, is increasingly reported in the zoonotic pathogen Streptococcus suis. Most of...
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Analysis of the Nasal Microbiota in Healthy and Diseased Pigs
Massive sequencing of a fragment of 16S rRNA gene allows the characterization of bacterial communities in different body sites: the microbiota. Nasal...
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Techniques for Genetic Manipulation
In the last few decades, molecular techniques and genetic modification have been used in genotype and phenotype studies of S. suis. Genomic...
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Setup of Aerosol Delivery System to Prevent Measles Virus Infection in Nonhuman Primate Model
Measles virus is one of the most contagious airborne human viruses which keeps causing outbreaks in numerous countries over the world despite the...
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A Semiquantitative Protein-Fragment Complementation Assay to Study Protein-Protein Interactions of the Polymerase Complex in Cellula
Protein-fragment complementation assays (PCAs) are powerful tools to investigate protein-protein interactions in a cellular context. These are...