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Setup of Aerosol Delivery System to Prevent Measles Virus Infection in Nonhuman Primate Model
Measles virus is one of the most contagious airborne human viruses which keeps causing outbreaks in numerous countries over the world despite the...
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Modeling HIV-1 Infection in CNS via Infected Monocytes Using Immunocompetent Brain Organoids
The development of 3D-organoid models has revolutionized the way diseases are studied. Recently, our brain organoid model has been shown to...
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T Cell Subsets and Immune Homeostasis
T cells are a heterogeneous group of cells that can be classified into different subtypes according to different classification methods. The body’s...
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Isolation and Single-Cell Transcriptomic Analysis of Murine Regulatory B Cells
Regulatory B (Breg) cells have been demonstrated to play an important role in the inhibition of a wide range of immunological responses, and they are...
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CRISPR-Cas9-Induced Gene Editing in Primary Human Monocytes
Monocytes play important and diverse roles in both homeostatic and inflammatory immune responses. The CRISPR-Cas9 system in lentiviral vectors has...
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Employing CRISPR-Cas9 to Enhance T Cell Effector Function
As part of the adaptive immune system, T cells are critical to maintain immune homeostasis. T cells provide protective immunity by killing infected...
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Allele Frequency Net Database
The allele frequency net database (AFND, http://www.allelefrequencies.net ) is an online web-based...
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NanoHLA: A Method for Human Leukocyte Antigen Class I Genes Ty** Without Error Correction Based on Nanopore Sequencing Data
Human leukocyte antigen (HLA) ty** is of great importance in clinical applications such as organ transplantation, blood transfusion, disease...
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Full-Length Characterization of Novel HLA-DRB1 Alleles for Reference Database Submission
The prerequisite for successful HLA genoty** is the integrity of the large allele reference database IPD-IMGT/HLA. Consequently, it is in the...
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A Semiquantitative Protein-Fragment Complementation Assay to Study Protein-Protein Interactions of the Polymerase Complex in Cellula
Protein-fragment complementation assays (PCAs) are powerful tools to investigate protein-protein interactions in a cellular context. These are...
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Single-Cell Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolution Scanning Analysis to Visualize the Reactivation of Latent HIV-1
Latent HIV-1 reservoirsHIV-1 reservoirs are a major obstacle to the eradication of HIV-1. Several cure strategies have been proposed to eliminate...
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Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues
Correlative light-electron microscopy (CLEM) has evolved in the last decades, especially after significant developments in sample preparation,...
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Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics
RNA viruses generate defective genomes naturally during virus replication. Defective genomes that interfere with the infection dynamics either...
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Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay
To integrate with host chromatin and establish a productive infection, HIV-1 must translocate the viral Ribonucleoprotein (RNP) complex through the...
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Measles Foci Reduction Neutralization Test (FRNT)
The plaque reduction neutralization test (PRNT) and the enzyme-linked immunosorbent assay (ELISA) are both widely used to assess immunity to...
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Multiplex Bead Assay for the Serological Surveillance of Measles and Rubella
There is increasing interest in evaluating antibody responses to multiple antigen targets in a single assay. Immunity to measles and rubella are...
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Laboratory Methods for Culture and Identification
This chapter addresses the cultivation, identification, and characterization of Streptococcus suis. Here, we describe in detail the most used...
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Detection and Quantification of Conjugative Transfer of Mobile Genetic Elements Carrying Antibiotic Resistance Genes
Multidrug resistance, due to acquired antimicrobial resistance genes, is increasingly reported in the zoonotic pathogen Streptococcus suis. Most of...
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Evaluation of the Economic Impact of Streptococcus suis-Associated Disease
The economic impact of Streptococcus suis-associated disease at farm level is well known by the producers, but the cost in a region or a country is...