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Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases
Polymerase chain reaction (PCR) is a common and indispensable technique that has been employed since its discovery for the diagnosis of infectious...
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Ligase Detection Reaction-Fluorescent Microsphere Assay
Disease outbreaks in swine production cause severe economic loss. Conventional diagnostic methods are laborious and less sensitive or time consuming....
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Metabolic Profile of Innate Immune Cells
Macrophages are immune cells broadly distributed in all tissues of the body and perform key roles in regulating inflammatory responses and tissue...
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Metabolic Pathways in Immune Cells Commitment and Fate
Cellular metabolism, with catabolic and anabolic pathways, is responsible to supply energy and essential molecules involved in cell growth and...
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Metabolic Reprogramming and Infectious Diseases
Cellular organization is possible through metabolic processes that ensure the construction of biomass and energy supply. Central metabolic pathways...
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Immunometabolism and Organ Transplantation
Inflammation fundamentally influences short and long-term performance of solid organ allografts. Thus, it is relevant to control inflammatory...
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Adipose Tissue and Immuno-Metabolic Regulation
Evolution has given rise to a variety of ways to store energy ranging from small intracellular lipid droplets to specialized tissue called adipose,...
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Overview of Immune Responses
The immune system comprises a network of humoral and cellular elements that recognizes self and non-self molecules or components through soluble and...
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Immune Regulation of Adipose Tissue Browning
Adipose tissue is composed by adipocytes and stromal-vascular cells that include a wide variety of immune cells. The proportion of these cells in the...
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Physical Exercise and Metabolic Reprogramming
In this chapter, we discuss the effects of acute and chronic exercise in metabolic reprograming. The exercise causes considerable systemic...
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Metabolic Pathways and Cell Signaling
This chapter overviews the basic features of metabolic pathways concerning the three main classes of energetic substrates – carbohydrates, lipids and...
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Obesity, Metabolic Syndrome and Inflammation
The pathophysiology underlying metabolic disease and Type 2 Diabetes (T2D) is a complex orchestration of deregulated metabolic pathways in multiple...
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Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective
Despite the newer techniques that offer rapid diagnosis of viral diseases by demonstration of viral antigen or viral nucleic acid in the specimens,...
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Antibody-Based Sensors for Pathogen Detection
Antibodies are soluble biomolecules of the Immunoglobulin family found in serum, which can specifically bind to and neutralize diverse antigens....
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Methods for Quantification of Viruses
Virus quantification is widely practised in both commercial and academic laboratories involved in research or production of viral vaccines,...
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Protocols for Isolation of Genetic Materials from RNA Viruses
The isolation of viral RNA with purity and integrity is a critical element for the overall success of viral diagnosis. The era of classical virology...
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An Overview of Mouse Monoclonal Antibody Production
Antibodies are an important tool in the field of diagnostics and therapeutics owing to their affinity to bind with specific antigen. These can be of...
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Production of Virus-like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis
Over many years, the baculovirus expression vector system (BEVS) has grown in popularity as a platform for expressing recombinant proteins. In...
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RNA-PAGE-Based Diagnosis of Viral Diseases
Regardless of low resources, many of the techniques have proven indispensable in every laboratory around the world, particularly in develo**...
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Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases
For the diagnosis of pathogens, amplification and detection of their genetic material is an essential step, but the traditional method of...