69 Result(s)
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Protocol
Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease
Interferon regulatory factor 3 (IRF-3) is a ubiquitously expressed latent cellular transcription factor that plays a pivotal role in control of innate, type I interferon (IFN) antiviral responses. After viral ...
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Protocol
Methodologies in Assaying Prostate Cancer Stem Cells
The cancer stem cell (CSC) theory posits that only a small population of tumor cells within the tumor has the ability to reinitiate tumor development and is responsible for tumor homeostasis and progression. T...
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Protocol
Development of Bacterial Vectors for Tumor-Targeted Gene Therapy
Gene therapy holds great promise for the treatment of cancer. The success of the strategy relies on effective gene transfer into tumor microenvironments. Although a variety of gene delivery vehicles, such as v...
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Protocol
Molecular Beacons for Protein—DNA Interaction Studies
Real-time monitoring of DNA—protein interactions involving molecular beacon (MB) and molecular beacon aptamer (MBA) was discussed in this chapter. MBs are single-stranded oligonucleotide probes with a hairpin ...
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Protocol
Size Fractionation of Cell-Free DNA in Maternal Plasma and Its Application in Noninvasive Detection of Fetal Single Gene Point Mutations
Recent studies have shown that cell-free fetal DNA in maternal plasma can be enriched by means of size fractionation. This technique makes use of the smaller size of fetal DNA fragments compared with maternal ...
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Protocol
In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis
Intermittent parathyroid hormone (PTH) administration increases bone formation and bone mass and is being used as a therapy for osteoporosis. By contrast, chronic hyperparathyroidism results in the metabolic b...
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Protocol
Genomic Analysis of Gene Expression of Staphylococcus aureus
The microarray has shown tremendous potential for investigating gene expression profiles and expression levels in comparative biology; exploring the regulation mechanisms of gene expression; and evaluating tar...
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Protocol
LINKAGE Programs
Identification of the genes for a human disease provides significant insights into the molecular mechanism underlying the pathogenesis of the disease. A human disease gene can be identified by its chromosomal ...
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Protocol
Positional Cloning
Positional cloning is a comprehensive genetic strategy used to identify a disease-causing gene without any prior knowledge of the pathogenesis or protein defects involved in the disease process. The basic proc...
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Protocol
Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies
Methods for generating recombinant vaccinia viruses for the expression of foreign viral glycoproteins in mammalian cell lines and the purification of expressed viral glycoproteins are described. These methods ...
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Protocol
Generation of Transgenic Mice for Cardiovascular Research
The transgenic mouse technology is a powerful tool that can be used for creating animal models for cardiovascular disease to identify molecular pathogenic mechanisms and for identifying the physiological funct...
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Protocol
Functional Analysis of the N-Linked Glycans Within the Fusion Protein of Respiratory Syncytial Virus
The respiratory syncytial virus fusion (F) protein is initially expressed as a single polypeptide chain (F0). The F0 subsequently undergoes posttranslational cleavage-by-cell protease activity to produce the F...
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Protocol
Markers of Apoptosis
Apoptosis is a physiological process that occurs in cells during development and normal cellular processes. The useless, unwanted, or damaged cells die during the apoptotic process. However, if signals instruc...
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Protocol
Targeted Somatic Mutagenesis in the Mouse Epidermis
The efficient introduction of somatic mutations in a given gene at a given time and in specific cell types of the skin will greatly facilitate the studies of a number of genes expressed in the skin and the pro...
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Protocol
FACS Enrichment of Human Keratinocyte Stem Cells
Recent work from our laboratory has led to the development and validation of fluorescence-activated cell sorting (FACS)-based techniques to prospectively isolate viable keratinocyte stem cells from both human ...
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Protocol
Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization
We have developed a sensitive method for the detection of specific genes simultaneously. First, DNA was amplified by a novel asymmetric multiplex PCR with universal primer(s). Second, the 6-carboxytetramethylr...
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Protocol
Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology
Congestive heart failure (CHF) is characterized by impaired cardiovascular reflexes and increased neurohumoral drive. The long-term sympatho-excitation increases the progression and risk of mortality during CH...
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Protocol
Construction of VNP20009
The engineering of tumor-targeting Salmonella strains bearing a high degree of both attenuation and tumor inhibition is a recently developed approach to vector-based treatment of cancer (1,2). Tumor-targeted Salm...
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Protocol
Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian Cells
The hepatitis B virus (HBV) C gene encodes the core protein and the precore protein. The core protein, also known as the core antigen, is an important serologic marker for HBV infection. Although this protein ...
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Protocol
In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage
Because of the limited availability of donor cartilage for resurfacing defects in articular surfaces, there is tremendous interest in the in vitro bioengineering of cartilage replacements for clinical applicat...
