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Li zhu
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Biotechnology
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Article
Open AccessHepatic small extracellular vesicles promote microvascular endothelial hyperpermeability during NAFLD via novel-miRNA-7
A recent study has reported that patients with nonalcoholic fatty liver disease (NAFLD) are more susceptible to coronary microvascular dysfunction (CMD), which may predict major adverse cardiac events. However...
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Open AccessProtective effects of sodium butyrate on rotavirus inducing endoplasmic reticulum stress-mediated apoptosis via PERK-eIF2α signaling pathway in IPEC-J2 cells
Rotavirus (RV) is a major pathogen that causes severe gastroenteritis in infants and young animals. Endoplasmic reticulum (ER) stress and subsequent apoptosis play pivotal role in virus infection. However, the...
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Change of soluble acid invertase gene (SAI-1) haplotype in hybrid sorghum breeding program in China
Sugar metabolism is the most important and core one which drives plant growth and development. Invertases are key enzymes that regulate sugar metabolism. A still-growing number of studies have revealed that in...
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Fasting Increases Iron Export by Modulating Ferroportin 1 Expression Through the Ghrelin/GHSR1α/MAPK Pathway in the Liver
The liver is contributed to maintaining body iron homeostasis and controlling of body adaptation to fasting. Although previous studies implied a negative relationship between iron and ghrelin in both mice and ...
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Open AccessManipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency
Deactivated Cas9 (dCas9) led to significant improvement of CRISPR/Cas9-based techniques because it can be fused with a variety of functional groups to form diverse molecular devices, which can manipulate or mo...
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Multi-stage glucose/pachymaran co-feeding enhanced endo-β-1,3-glucanase production by Trichoderma harzianum via simultaneous increases in cell concentration and inductive effect
Endo-β-1,3-glucanase is used to hydrolyze curdlan in a wide range of oligosaccharides production processes. Using pachymaran as the sole carbon source resulted in an endo-β-1,3-glucanase activity of 86.1 U/mL ...
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Open AccessSoft - sensing modeling based on ABC - MLSSVM inversion for marine low - temperature alkaline protease MP fermentation process
Aiming at the characteristics of nonlinear, multi-parameter, strong coupling and difficulty in direct on-line measurement of key biological parameters of marine low-temperature protease fermentation process, a...
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Enhanced N-acetyl-d-neuraminic production from glycerol and N-acetyl-d-glucosamine by metabolically engineered Escherichia coli with a two-stage pH-shift control strategy
Typical N-acetyl-d-neuraminic acid (Neu5Ac) production uses N-acetyl-d-glucosamine (GlcNAc) and excess pyruvate as substrates in the enzymatic or whole-cell biocatalysis process. In a previous study, a Neu5Ac-pro...
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Article
Increased productivity of l-2-aminobutyric acid and total turnover number of NAD+/NADH in a one-pot system through enhanced thermostability of l-threonine deaminase
To strengthen NADH regeneration in the biosynthesis of l-2-aminobutyric acid (l-ABA).
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Expression profile of long non-coding RNAs during the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocyte-like cells
We aimed to investigate the differentially expressed long non-coding RNAs (lncRNAs) during the differentiation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) into cardiomyocyte-like cells ind...
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Production of rhamnolipids by semi-solid-state fermentation with Pseudomonas aeruginosa RG18 for heavy metal desorption
Foaming problem and cost of substrate limit the commercial application of rhamnolipids, a potential biosurfactant produced by Pseudomonas aeruginosa. We explored the production of rhamnolipids by a semi-solid-sta...
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Different nitrogen sources change the transcriptome of welan gum-producing strain Sphingomonas sp. ATCC 31555
To reveal effects of different nitrogen sources on the expressions and functions of genes in Sphingomonas sp. ATCC 31555, it was cultivated in medium containing inorganic nitrogen (IN), organic nitrogen (ON), or ...
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Article
Production of β-carotene by expressing a heterologous multifunctional carotene synthase in Yarrowia lipolytica
To obtain functional expression of a heterologous multifunctional carotene synthase containing phytoene synthase, phytoene dehydrogenase, and lycopene β-cyclase activities encoded by carS from Schizochytrium sp. ...
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Phosphoenolpyruvate-supply module in Escherichia coli improves N-acetyl-d-neuraminic acid biocatalysis
N-Acetyl-d-neuraminic acid (Neu5Ac) is often synthesized from exogenous N-acetylglucosamine (GlcNAc) and excess pyruvate. We have previously constructed a recombinant Escherichia coli ...
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Discovery of the New Plant Growth-Regulating Compound LYXLF2 Based on Manipulating the Halogenase in Amycolatopsis orientalis
Analysis of the Amycolatopsis orientalis HCCB10007 genome revealed new gene clusters involved in natural product biosynthesis that were not associated with the production of known compounds. Halogenases are a typ...
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Inhibitor of p53–p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells
P53 is shown recently to play an important role in the proliferation and differentiation of mesenchymal stem cells. In this study, human umbilical cord derived mesenchymal stem cells (hUCMSCs) were isolated an...
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Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system
Yarrowia lipolytica is categorized as a generally recognized as safe (GRAS) organism and is a heavily documented, unconventional yeast that has been widely incorporated into multiple indu...
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Expression and Significance of DLL4-–Notch Signaling Pathway in the Differentiation of Human Umbilical Cord Derived Mesenchymal Stem Cells into Cardiomyocytes Induced by 5-Azacytidine
hUCMSCs were isolated and purified from the umbilical cords of normal or cesarean term deliveries under sterile conditions. Flow cytometry analysis revealed that CD13, CD29, CD44, CD90, and CD105 were highly e...
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One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica
Yarrowia lipolytica is an unconventional yeast, and is generally recognized as safe (GRAS). It provides a versatile fermentation platform that is used commercially to produce many added-value pro...
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Fine map** of a dominant thermo-sensitive genic male sterility gene (BntsMs) in rapeseed (Brassica napus) with AFLP- and Brassica rapa-derived PCR markers
A new thermo-sensitive dominant genic male sterility (TSDGMS) line of Brassica napus was found and mapped in this paper. Our result will greatly accelerate t...
