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  1. No Access

    Article

    Binding, tuning and mechanical function of the 4-hydroxy-cinnamic acid chromophore in photoactive yellow protein

    The bacterial photoreceptor protein photoactive yellow protein (PYP) covalently binds the chromophore 4-hydroxy coumaric acid, tuning (spectral) characteristics of this cofactor. Here, we study this binding an...

    Michael A. van der Horst, Jos C. Arents… in Photochemical & Photobiological Sciences (2007)

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    Chapter

    Light-induced behavioral responses (‘phototaxis’) in prokaryotes

    Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...

    Judith P. Armitage, Klaas J. Hellingwerf in Discoveries in Photosynthesis (2005)

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    Article

    Chromophore composition of a heterologously expressed BLUF-domain

    Upon heterologous expression of the BLUF (for: Blue-Light sensing Using Flavin) domain from AppA, a transcriptional anti-repressor from Rhodobacter sphaeroides, in Escherichia coli, photoactive holo-protein is fo...

    Wouter Laan, Teresa Bednarz, Joachim Heberle… in Photochemical & Photobiological Sciences (2004)

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    Article

    Early molecular events in the photoactive yellow protein: role of the chromophore photophysics

    We report a comparative study of the isomerization reaction in native and denatured photoactive yellow protein (PYP) and in various chromophore analogues in their trans deprotonated form. The excited-state relaxa...

    Pascale Changenet-Barret, Agathe Espagne… in Photochemical & Photobiological Sciences (2004)

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    Article

    Tryptophan fluorescence monitors structural changes accompanying signalling state formation in the photocycle of photoactive yellow protein

    Photoactive yellow protein, a small, water-soluble blue-light absorbing photoreceptor protein from Ectothiorhodospira (Halorhodospira) halophila has a structure with two hydrophobic cores, of which the main one h...

    Thomas Gensch, Johnny Hendriks… in Photochemical & Photobiological Sciences (2004)

  6. No Access

    Article

    Light-induced behavioral responses (`phototaxis') in prokaryotes

    Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...

    Judith P. Armitage, Klaas J. Hellingwerf in Photosynthesis Research (2003)

  7. No Access

    Article

    The molecular basis of sensing and responding to light in microorganisms

    Photon absorption in biological signal transfer is mediated by a limited number of photoreceptor families, each characterised by binding of chromophore from a particular class of chemical compounds. Most photo...

    Klaas J. Hellingwerf in Antonie van Leeuwenhoek (2002)

  8. No Access

    Article

    The Metabolic Response of Saccharomyces Cerevisiae to Continuous Heat Stress

    A study has been initiated to integrate molecular and physiological responses of Saccharomyces cerevisiae to heat stress conditions. We focus our research on a quantification of the energetics of the stress respo...

    Femke I.C. Mensonides, J. Merijn Schuurmans… in Molecular Biology Reports (2002)

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    Article

    Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222

    Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP398) into one absorbing at 483 n...

    Jasper J. van Thor, Thomas Gensch, Klaas J. Hellingwerf… in Nature Structural Biology (2002)

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    Protocol

    8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Protein

    Fluorescence resonance energy transfer (FRET) is a technique that can be used to estimate intermolecular distances between pigment molecules, which is an approach first proposed by Stryer (1). The theoretical bas...

    Jasper van J. Thor, Klaas J. Hellingwerf in Green Fluorescent Protein (2002)

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    Article

    Alkalispirillum mobile gen. nov., spec. nov., an alkaliphilic non-phototrophic member of the Ectothiorhodospiraceae

    From cultures of the anoxygenic phototroph Halorhodospira halophila SL-1, an aerobic, gram-negative spirillum was isolated. This moderately halophilic, alkaliphilic bacterium was motile by means of a single pola...

    Micha J. Rijkenberg, Remco Kort, Klaas J. Hellingwerf in Archives of Microbiology (2001)

  12. No Access

    Chapter

    Sensing of Green Light in Complementary Chromatic Adaptation of the Cyanobacterium Calothrix sp.

    Light harvesting is essential for phototrophic organisms. The reaction centers of PS2 and PS1 in cyanobacteria contain chl a, † a ubiquitous pigment that absorbs light in two spectral domains. In the blue, absorp...

    Hans C. P. Matthijs, Jeroen H. Geerdink, Hans Balke… in The Phototrophic Prokaryotes (1999)

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    Article

    Current topics in signal transduction in bacteria

    Among the signal transfer systems in bacteria two types predominate: two-component regulatory systems and quorum sensing systems. Both types of system can mediate signal transfer across the bacterial cell enve...

    Klaas J. Hellingwerf, Wim C. Crielaard… in Antonie van Leeuwenhoek (1998)

  14. Article

    Characterization and transcriptional regulation of the Synechocystis PCC 6803, petH gene, encoding ferredoxin-NADP+ oxidoreductase: involvement of a novel type of divergent operator

    Jasper J. van Thor, Klaas J. Hellingwerf, Hans C.P. Matthijs in Plant Molecular Biology (1998)

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    Article

    Structural and dynamic changes of photoactive yellow protein during its photocycle in solution

    Light irradiation of photoactive yellow protein (PYP) induces a photocycle, in which red-shifted (pR) and blue-shifted (pB) intermediates have been characterized. An NMR study of the long-lived pB intermediate...

    Gilles Rubinstenn, Geerten W. Vuister, Frans A. A. Mulder… in Nature Structural Biology (1998)

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    Article

    Characterization and transcriptional regulation of the Synechocystis PCC 6803 petH gene, encoding ferredoxin-NADP+ oxidoreductase: involvement of a novel type of divergent operator

    The petH gene, encoding ferredoxin-NADP+ oxidoreductase (FNR), has been characterised in the unicellular cyanobacterium Synechocystis PCC 6803. Its product, FNR, was heterologously produced and functionally chara...

    Jasper J. van Thor, Klaas J. Hellingwerf, Hans C.P. Matthijs in Plant Molecular Biology (1998)

  17. No Access

    Chapter

    Global bioenergetics

    The surface of the earth is subject to a continuous input of free enthalpy originating in the sun. A small, but important part of this free enthalpy is diverted, transiently, to physical chemical structures th...

    Hans V. Westerhoff, Wim Crielaard, Klaas J. Hellingwerf in Bioenergetics (1997)

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    Article

    Effect of nitrogen sources on the regulation of extracellular lipase production in Acinetobacter calcoaceticus strains

    Extracellular lipase production and lipA expression were studied in A.calcoaceticus strains in relation to the nitrogen sources. Addition of casamino-acids or tryptone improved 2–3 times lipase yield as compared ...

    Alfredo Cordenons, Rodrigo Gonzalez, Ruben Kok… in Biotechnology Letters (1996)

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    Article

    Acinetobacter calcoaceticus liberates chromosomal DNA during induction of competence by cell lysis

    A transformation assay was used to assay the amount of DNA present in the extracellular medium of a growing culture of Acinetobacter calcoaceticus. It was observed that small amounts of DNA were liberated during ...

    Ronald Palmen, Klaas J. Hellingwerf in Current Microbiology (1995)

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    Article

    Physiological regulation of competence induction for natural transformation in Acinetobacter calcoaceticus

    Acinetobacter calcoaceticus induced competence for natural transformation maximally after dilution of a stationary culture into fresh medium. Competence was gradually lost during prolonged exponential growth and...

    Ronald Palmen, Pieter Buijsman, Klaas J. Hellingwerf in Archives of Microbiology (1994)

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