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Article
Open AccessComparative transcriptomic analysis of silkwormBmovo-1 and wild type silkworm ovary
The detailed molecular mechanism of Bmovo-1 regulation of ovary size is unclear. To uncover the mechanism of Bmovo-1 regulation of ovarian development and oogenesis using RNA-Seq, we compared the transcriptomes o...
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Article
Transcriptome analysis of BmN cells following over-expression of BmSTAT
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway are involved in immune response, cell proliferation, differentiation, cell migration and apoptosis. In order to ...
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Article
Open AccessDevelo** a mesophilic co-culture for direct conversion of cellulose to butanol in consolidated bioprocess
Consolidated bioprocessing (CBP) of butanol production from cellulosic biomass is a promising strategy for cost saving compared to other processes featuring dedicated cellulase production. CBP requires microbi...
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Article
The gene expression profile of resistant and susceptible Bombyx mori strains reveals cypovirus-associated variations in host gene transcript levels
High-throughput paired-end RNA sequencing (RNA-Seq) was performed to investigate the gene expression profile of a susceptible Bombyx mori strain, Lan5, and a resistant B. mori strain, Ou17, which were both orally...
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Article
Construction of transgenic silkworm spinning antibacterial silk with fluorescence
A targeting vector consisting of a fusion gene of the green fluorescent protein (GFP) gene gfp and the antimicrobial peptide cecropin gene cec flanked by pieces of the 5′ and 3′ sequences of the fibroin L chain g...
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Article
Construction of transformed, cultured silkworm cells and transgenic silkworm using the site-specific integrase system from phage φC31
The Streptomyces bacteriophage, φC31, uses a site-specific integrase enzyme to perform efficient recombination. The recombination system uses specific sequences to integrate exogenous DNA from the phage into a ho...
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Article
Alternative splicing, expression patterns and promoter characters of vasa-like gene from the silkworm, Bombyx mori
VASA is considered to be one of the most reliable molecular marker of germ cells. In order to study the Bombyx mori vasa-like gene (Bmvlg), the cDNAs of Bmvlg were cloned and sequenced, and the re...
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Article
Expression of hGM-CSF in silk glands of transgenic silkworms using gene targeting vector
The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human granucyto-macrophage colony-stimulating factor (hGM-CSF) in the...
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Article
Expression of UreB and HspA of Helicobacter pylori in silkworm pupae and identification of its immunogenicity
For mass production of urease B subunit (UreB) and heat shock protein A subunit (HspA) of Helicobacter pylori with Bombyx mori nuclear polyhedrosis virus (BmNPV) baculovirus expression system (BES) and to determi...
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Article
Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells
The expression of the human insulin-like growth factor (hIGF-I) gene driven by the Fhx/P25 promoter in the silk glands of transgenic silkworms (Bombyx mori) and in transformed silkworm cells, was achieved using B...
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Article
Biological hydrogen production from organic wastewater by dark fermentation in China: Overview and prospects
Biological hydrogen production by dark fermentation is an important part of biological hydrogen production technologies. China is a typical develo** country that heavily relies on fossil fuels; thus, new, cl...
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Article
Expression of hIGF-I in the silk glands of transgenic silkworms and in transformed silkworm cells
To express human insulin-like growth factor-I (hIGF-I) in transformed Bombyx mori cultured cells and silk glands, the transgenic vector pigA3GFP-hIGF-ie-neo was constructed with a neomycin resistance gene driven ...
