Definition
Computational systems bioinformatics for RNAi screening and therapeutics is defined as complete computational workflow applicable to the hypothesis generation from large-scale data from image-based RNAi screenings as well as the improvement of RNAi-based therapeutics; the workflow includes automatic image analysis compatible to large-scale cell image data, together with unbiased statistical analysis and gene function annotation.
Introduction
RNA interference (RNAi) defines the phenomenon of small RNA molecules binding to its complementary sequence in certain messenger RNA, recruiting a specific protein complex to dissect the whole mRNA and thus silencing the expression of the corresponding gene. It is a highly conserved system within living cells to quantitatively control the activity of genes. In 1998, Fire et al. first clarified the causality of this phenomenon and named it as RNAi [1],...
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References
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Yin, Z., Fan, Y., Wong, S.T. (2016). Computational Systems Bioinformatics for RNAi. In: Bhushan, B. (eds) Encyclopedia of Nanotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9780-1_103
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DOI: https://doi.org/10.1007/978-94-017-9780-1_103
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