Definition
Microcell-mediated chromosome transfer (MMCT) is a method of transfer of intact or truncated chromosomes from a donor cell to a recipient cell through microcell fusion and hybrid cell selection. A microcell hybrid stably maintains the additional genetic material as functioning units within the cells. This technique is primarily used for transfer of a single donor chromosome (monochromosome transfer) of interest into a recipient cell.
Characteristics
A method of mammalian gene transfer, termed microcell-mediated chromosome transfer (MMCT), allows the stable introduction of exogenous chromosomal material from a donor cell into a recipient cell. MMCT is generally used to transfer a portion of the genetic information (generally one chromosome) from one cell to another. This procedure is used for genetics, gene map**, and gene expression/regulation studies in mammalian cells. One or more intact or truncated chromosomes from the donor cell can be transferred into the recipient...
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References
Dowdy SF, Scanlon DJ, Fasching CL et al (1990) Irradiation microcell-mediated chromosome transfer (XMMCT): the generation of specific chromosomal arm deletions. Genes Chromosomes Cancer 2:319–327
Fournier REK, Ruddle FH (1977) Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells. Proc Natl Acad Sci U S A 74:319–323
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Saxon PJ, Srivatsan ES, Leipzig GV et al (1985) Selective transfer of individual human chromosomes to recipient cells. Mol Cell Biol 5:140–146
Stanbridge EJ, Dowdy S, Bader S et al (1991) Monochromosome transfer provides functional evidence for tumor suppressor genes. In: Brugge J, Curran T, Harlow E, McCormick F (eds) Origins of human cancer: a comprehensive review. Cold Spring Harbor Laboratory Press, New York, pp 393–401
See Also
(2012) Enucleate. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, p 1259. doi:10.1007/978-3-642-16483-5_1914
(2012) FISH. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, pp 1415–1416. doi:10.1007/978-3-642-16483-5_2197
(2012) HAT. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, pp 1632–1633. doi:10.1007/978-3-642-16483-5_2573
(2012) Heterokaryon. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, p 1689. doi:10.1007/978-3-642-16483-5_2697
(2012) Karyoplast. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, p 1941. doi:10.1007/978-3-642-16483-5_3199
(2012) Microcell. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, p 2292. doi:10.1007/978-3-642-16483-5_3714
(2012) Microcell Hybrid. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, p 2292. doi:10.1007/978-3-642-16483-5_3715
(2012) Micronucleus. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, p 2300. doi:10.1007/978-3-642-16483-5_3726
(2012) Senescence. In: Schwab M (ed) Encyclopedia of Cancer, 3rd edn. Springer Berlin Heidelberg, p 3370. doi:10.1007/978-3-642-16483-5_5236
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Lung, M.L. (2015). Microcell-Mediated Chromosome Transfer. In: Schwab, M. (eds) Encyclopedia of Cancer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27841-9_3716-2
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DOI: https://doi.org/10.1007/978-3-642-27841-9_3716-2
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