Abstract:
A number of microorganisms exhibit the unusual property of being tolerant to high concentrations of organic solvents such as toluene, xylenes, ethylbenzene, 1-octanol, 1-decanol, etc. in the culture medium. These microbes are of utmost importance in industrial processes associated with biotransformations in double-phase systems and bioremediation. In this study we describe some of the tools that can be used to understand the physiological basis underlying solvent tolerance; information that can, in turn, be used to expand the range of industrial microorganisms that exhibit these properties.
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References
Abril MA, Michán C, Timmis KN, Ramos JL (1989) Regulator and enzyme specificities of the TOL plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway. J Bacteriol 171: 6782–6790.
Bernal P, Muñoz-Rojas J, Hurtado A, Ramos JL, Segura A (2007) A Pseudomonas putida cardiolipin synthesis mutant exhibits increased sensitivity to drugs related to transport functionality. Environ Microbiol 9: 1135–1145.
de Lorenzo V, Herrero M, Jakubzik U, Timmis KN (1990) Mini-Tn5 transposon derivatives for insertion mutagenesis promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol 172: 6568–6572.
Dominguez-Cuevas P, González-Pastor JE, Marqués S, Ramos JL, de Lorenzo V (2006) Transcriptional tradeoff between metabolic and stress-response programs in Pseudomonas putida KT2440 cells exposed to toluene. J Biol Chem 281: 11981–11991.
Duque E, Rodriguez-Hervá JJ, de la Torre J, Domínguez-Cuevas P, Muñoz-Rojas J, Ramos JL (2007) The RpoT regulon of Pseudomonas putida DOT-T1E and its role in stress endurance against solvents. J Bacteriol 189: 207–219.
Enderle PJ, Farwell MA (1998) Electroporation of freshly plated Escherichia coli and Pseudomonas aeruginosa cells. Biotechniques 25: 954–958.
Espinosa-Urgel M, Salido A, Ramos JL (2000) Genetic analysis of functions involved in adhesion of Pseudomonas putida to seeds. J Bacteriol 182: 2363–2369.
Heipieper HJ, Diefenbach R, Keweloh H (1992) Conversion of cis unsaturated fatty acids to trans, a possible mechanism for the protection of phenol degrading Pseudomonas putida P8 from substrate toxicity. Appl Environ Microbiol 58: 1847–1852.
Herrero M, de Lorenzo V, Timmis KN (1990) Transposon vectors containing nonantibiotic resistance selection markers for cloning and stable chromosomal insertion of foreing genes in gram-negative bacteria. J Bacteriol 172: 6557–6567.
Huertas MJ, Duque E, Rosselló-Mora R, Mosqueda G, Godoy P, Christensen B, Molin S, Ramos JL (2000) Tolerance to sudden organic solvent shocks by soil bacteria and characterization of Pseudomonas putida strains isolated from toluene polluted sites. Environ Sci Technol 34: 3395–3400.
Inoue A, Horikoshi K (1989) A Pseudomonas thrives in high concentrations of toluene. Nature 338: 264–266.
Isken S, de Bont JAM (1996) Active efflux of toluene in a solvent-resistant bacterium. J Bacteriol 178: 6056–6058.
Junker F, Ramos JL (1999) Involvement of the cis-trans isomerase CtiT1 in solvent resistance in Pseudomonas putida DOT-T1. J Bacteriol 181: 5693–5700.
Kaniga K, Delor I, Cornelis GR (1991) A wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene 20: 137–141.
Keweloh H, Heipieper HJ (1996) Trans unsaturated fatty acids in bacteria. Lipids 31: 129–137.
Kieboom J, Dennis JJ, de Bont JAM, Zylstra GJ (1998) Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance. J Biol Chem 273: 85–91.
Llamas MA, Rodriguez-Hervá JJ, Hancock REW, Bitter W, Tommassen J, Ramos JL (2003) Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane. J Bacteriol 185: 4707–4716.
Meyer D, Witholt B, Schmid A (2005) Suitability of recombinant Eschericha coli and Pseudmonas putida strains for selective biotransformation of m-nitrotoluene by xylene monooxygenase. Appl Environ Microbiol 71: 6624–6632.
Mosqueda G, Ramos-Gonzalez MI, Ramos JL (1999) Toluene metabolism by the solvent-tolerant Pseudomonas putida DOT-T1 strain, and its role in solvent impermeabilization. Gene 232: 69–76.
Neumann G, Cornelissen S, van Breukelen F, Hunger S, Lippold H, Loffhagen N, Wick LY, Heipieper HJ (2006) Energetics and surface properties of Pseudomonas putida DOT-T1E in a two-phase fermentation system with 1-decanol as second phase. Appl Environ Microbiol 72: 4232–4238.
Prentki P, Krisch HM (1984) In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29: 303–313.
Ramos JL, Duque E, Huertas MJ, Haidour A (1995) Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons. J Bacteriol 177: 3911–3916.
Ramos JL, Duque E, Rodriguez-Hervá JJ, Godoy P, Haïdour A, Reyes F, Fernández-Barrero A (1997) Mechanisms for solvent tolerance in bacteria. J Biol Chem 272: 3887–3890.
Ramos JL, Duque E, Godoy P, Segura A (1998) Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E. J Bacteriol 180: 3323–3329.
Ramos JL, Duque E, Gallegos MT, Godoy P, Ramos-González MI, Rojas A, Terán W, Segura A (2002) Mechanisms of solvent tolerance in gram-negative bacteria. Annu Rev Microbiol 56: 743–768.
Ramos-González M-I, Ben-Bassat A, Campos M-J, Ramos JL (2003) Genetic engineering of a highly solvent-tolerant Pseudomonas putida strain for biotransformation of toluene to p-hydroxybenzoate. Appl Environ Microbiol 69: 5120–5127.
Rojas A, Duque E, Mosqueda G, Golden G, Hurtado A, Ramos JL, Segura A (2001) Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E. J Bacteriol 183: 3967–3973.
Rojas A, Duque E, Schmid A, Hurtado A, Ramos JL, Segura A (2004) Biotransformation in double-phase systems: physiological responses of Pseudomonas putida DOT-T1E to a double phase made of aliphatic alcohols and biosynthesis of substituted catechols. Appl Environ Microbiol 70: 3637–3643.
Segura A, Rojas A, Hurtado A, Huertas MJ, Ramos JL (2003) Comparative genomic analysis of solvent extrusion pumps in Pseudomonas strains exhibiting different degrees of solvent tolerance. Extremophiles 7: 371–376.
Segura A, Godoy P, van Dillewijn P, Hurtado A, Arroyo N, Santacruz S, Ramos JL (2005) Proteomic analysis reveals the participation of energy- and stress-related proteins in the response of Pseudomonas putida DOT-T1E to toluene. J Bacteriol 187: 5937–5945.
Sikkema J, de Bont JAM, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59: 201–222.
Volkers RJM, de Jong AL, Hulst AG, van Baar BLM, de Bont JAM, Wery J (2006) Chemostat-based proteomic analysis of toluene-affected Pseudomonas putida S12. Environ Microbiol 8: 1674–1679.
Weber FJ, Isken S, de Bont JAM (1994) Cis/trans isomerization of fally acids as a defence mechanism of Pseudomonas putida strains to toxic concentrations of toluene. Microbiology 140: 2013–2017.
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Duque, E. et al. (2010). Protocols for the Characterization of Solvent Tolerant Microorganisms: Construction and Characterization of Mutants . In: Timmis, K.N. (eds) Handbook of Hydrocarbon and Lipid Microbiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77587-4_309
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DOI: https://doi.org/10.1007/978-3-540-77587-4_309
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