Abstract
Latent membrane protein 2A (LMP-2A) of Epstein-Barr virus (EBV) mimics a constitutively active B-cell receptor (BCR) and plays a key role in viral latency and EBV pathogenesis. By functioning as a BCR mimic, LMP-2A drives B-cell development, resulting in the bypass of normal B-cell developmental checkpoints. To assess the function of LMP-2A, we have utilized a colony formation assay for the progenitor B cells that uses the B-cell proliferation factor IL-7.
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© 2005 Humana Press Inc.
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Ikeda, M., Longnecker, R. (2005). Pre-B-Cell Colony Formation Assay. In: Lieberman, P.M. (eds) DNA Viruses. Methods in Molecular Biology, vol 292. Humana Press. https://doi.org/10.1385/1-59259-848-X:279
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DOI: https://doi.org/10.1385/1-59259-848-X:279
Publisher Name: Humana Press
Print ISBN: 978-1-58829-353-4
Online ISBN: 978-1-59259-848-9
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