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23 Endonuclease G Isolation and Assays

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Mitochondrial DNA

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 197))

Abstract

Mitochondria in mammalian cells contain a nuclearly encoded, Mg+2 (Mn+2)-dependent DNA endonuclease activity (reviewed in ref. 1), that has been termed Endonuclease G (or Endo G) (2). This enzyme activity, an approx 60-kDa homodimer of a 29-kDa polypeptide, accounts for nearly all of the potent DNA endonuclease activity identified in crude protein extracts of isolated mitochondria (3). The 1133-bp (base pairs) cDNA for the human Endonuclease G gene has been mapped to chromosome 9q34.1 (4).

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Author information

Authors and Affiliations

  1. Department of Pathology, University of Colorado Health Sciences Center, Denver, CO

    Robert L. Low

  2. Division of Heart and Vascular Diseases, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD

    Mariana Gerschenson

Authors
  1. Robert L. Low
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  2. Mariana Gerschenson
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Editor information

Editors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC

    William C. Copeland

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© 2002 Humana Press Inc.

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Low, R.L., Gerschenson, M. (2002). 23 Endonuclease G Isolation and Assays. In: Copeland, W.C. (eds) Mitochondrial DNA. Methods in Molecular Biology™, vol 197. Humana Press. https://doi.org/10.1385/1-59259-284-8:331

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  • DOI: https://doi.org/10.1385/1-59259-284-8:331

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-972-8

  • Online ISBN: 978-1-59259-284-5

  • eBook Packages: Springer Protocols

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