Abstract
The purity and integrity of Isolated RNA IS a critical determinant of its effectiveness in such molecular biological procedures as Northern blot, poly A+ RNA separation, cDNA synthesis, and in vitro transcription and translation The successful isolation of total RNA from tissues or cell lines by any procedure involves four major steps: complete disruption of the cells or tissues; effective denaturation of the nuclear protein complex; inactivation of endogenous ribonuclease (RNase) activity; and purification of the RNA from the contaminating DNA, protein, and carbohydrates.
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References
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© 1998 Humana Press Inc.
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Mukhopadhyay, T., Roth, J.A. (1998). Isolation of Total RNA from Tissues or Cell Lines. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:55
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DOI: https://doi.org/10.1385/0-89603-494-1:55
Publisher Name: Humana Press
Print ISBN: 978-0-89603-494-5
Online ISBN: 978-1-59259-570-9
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