Abstract
Recombinant and native proteins of Pichia pastoris can be O-mannosylated on serine and threonine residues, allowing further elongation reactions to generate short O-linked oligosaccharides of mannose. Methods for release from the protein with alkaline β-elimination with or without reduction of the released saccharides, and for subsequent chromatographic and enzymatic characterization of these saccharides are described.
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Bretthauer, R.K. (2007). Characterization of O-Linked Saccharides on Glycoproteins. In: Cregg, J.M. (eds) Pichia Protocols. Methods in Molecular Biology, vol 389. Humana Press. https://doi.org/10.1007/978-1-59745-456-8_8
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DOI: https://doi.org/10.1007/978-1-59745-456-8_8
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