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Vectors and Strains for Expression

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Pichia Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 389))

Abstract

Selection of both an appropriate expression vector and corresponding strain is crucial for successful expression of heterologous proteins in Pichia pastoris. This chapter explores both the standard and new vector/strain options available for protein expression in this yeast. Incorporated into expression vectors are selectable markers based on biosynthetic pathway genes, dominant drug resistance, or the P. pastoris formaldehyde dehydrogenase gene (FLD1). Novel strains available for expression include those that increase secretion of heterologous protein by overexpressing eukaryotic protein disulfide isomerase, and those that decrease hyperglycosylation or provide human-type glycosylation. This chapter also discusses methods to create multicopy strains that will potentially provide optimized expression of recombinant proteins in P. pastoris.

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Authors and Affiliations

  1. Department of Biological Sciences, University of the Pacific, Stockton, CA

    Joan Lin-Cereghino & Geoff P. Lin-Cereghino

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  1. Joan Lin-Cereghino
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  2. Geoff P. Lin-Cereghino
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Editors and Affiliations

  1. Keck Graduate Institute of Applied Life Sciences, Claremont, CA

    James M. Cregg

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© 2007 Humana Press Inc., Totowa, NJ

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Lin-Cereghino, J., Lin-Cereghino, G.P. (2007). Vectors and Strains for Expression. In: Cregg, J.M. (eds) Pichia Protocols. Methods in Molecular Biology, vol 389. Humana Press. https://doi.org/10.1007/978-1-59745-456-8_2

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  • DOI: https://doi.org/10.1007/978-1-59745-456-8_2

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-429-6

  • Online ISBN: 978-1-59745-456-8

  • eBook Packages: Springer Protocols

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