Abstract
An unmet need exists for a clinical diagnostic to determine preterm birth (PTB) risk. Such an assessment is possible with high sensitivity and specificity using a panel of nine biomarkers. An integrated microfluidic analysis system for these biomarkers is being developed which includes microchip electrophoresis (μCE) separation. A t-shaped microchip device can be used to test the μCE portion of this integrated system to find appropriate separation conditions. These t-shaped microchips can be fabricated using photolithographically patterned Si templates and hot embossing. PTB biomarkers can be fluorescently labeled using an amine-reactive dye prior to μCE. The μCE conditions established using this t-shaped device should be useful in develo** a complete integrated microfluidic system for PTB risk assessment.
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Nielsen, A.V., Woolley, A.T. (2019). Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis. In: Phillips, T.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 1972. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9213-3_12
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DOI: https://doi.org/10.1007/978-1-4939-9213-3_12
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9213-3
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