Imaging the Lymph Node Stroma

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Intravital Imaging of Dynamic Bone and Immune Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1763))

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Abstract

Lymph node (LN) stromal cells are being recognized as key organizers of the immune system. They assemble in complex 3D networks and hence, need to be studied in situ to fully understand their exact functions. Here, we describe two distinct but complementary procedures that allow analyzing LN stromal cells at high resolution by confocal imaging.

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Acknowledgements

We would like to thank Delphine Suerinck for technical help. This work was supported by a grant from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program grant agreement N° 647384- STROMA. The authors declare no conflict of interest.

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Correspondence to Marc Bajénoff .

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Ghigo, C., Gentek, R., Bajénoff, M. (2018). Imaging the Lymph Node Stroma. In: Ishii, M. (eds) Intravital Imaging of Dynamic Bone and Immune Systems . Methods in Molecular Biology, vol 1763. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7762-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7762-8_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7761-1

  • Online ISBN: 978-1-4939-7762-8

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