Kidney Imaging: Intravital Microscopy

  • Protocol
  • First Online:
Intravital Imaging of Dynamic Bone and Immune Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1763))

Abstract

Intravital two-photon microscopy is a powerful imaging tool for investigating various biological processes in live animals. This chapter describes an overview of intravital imaging of the rodent kidney including animal surgery, characteristics of renal tubular autofluorescence, in vivo use of fluorescent probes, and renal immune-cell tracking.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Torres R, Velazquez H, Chang JJ, Levene MJ, Moeckel G, Desir GV, Safirstein R (2016) Three-dimensional morphology by multiphoton microscopy with clearing in a model of cisplatin-induced CKD. J Am Soc Nephrol 27(4):1102–1112

    Article  CAS  PubMed  Google Scholar 

  2. Unnersjo-Jess D, Scott L, Blom H, Brismar H (2016) Super-resolution stimulated emission depletion imaging of slit diaphragm proteins in optically cleared kidney tissue. Kidney Int 89(1):243–247

    Article  PubMed  Google Scholar 

  3. Dunn KW, Sandoval RM, Kelly KJ, Dagher PC, Tanner GA, Atkinson SJ, Bacallao RL, Molitoris BA (2002) Functional studies of the kidney of living animals using multicolor two-photon microscopy. Am J Physiol Cell Physiol 283(3):C905–C916

    Article  CAS  PubMed  Google Scholar 

  4. Peti-Peterdi J, Kidokoro K, Riquier-Brison A (2015) Novel in vivo techniques to visualize kidney anatomy and function. Kidney Int 88(1):44–51

    Article  PubMed  PubMed Central  Google Scholar 

  5. Nakano D, Doi K, Kitamura H, Kuwabara T, Mori K, Mukoyama M, Nishiyama A (2015) Reduction of tubular flow rate as a mechanism of oliguria in the early phase of endotoxemia revealed by intravital imaging. J Am Soc Nephrol 26(12):3035–3044

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Oberbarnscheidt MH, Zeng Q, Li Q, Dai H, Williams AL, Shlomchik WD, Rothstein DM, Lakkis FG (2014) Non-self recognition by monocytes initiates allograft rejection. J Clin Invest 124(8):3579–3589

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Hall AM, Crawford C, Unwin RJ, Duchen MR, Peppiatt-Wildman CM (2011) Multiphoton imaging of the functioning kidney. J Am Soc Nephrol 22(7):1297–1304

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Triantafilou K, Triantafilou M, Fernandez N (2000) Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies. Cytometry 41(4):316–320

    Article  CAS  PubMed  Google Scholar 

  9. Sandoval RM, Wagner MC, Patel M, Campos-Bilderback SB, Rhodes GJ, Wang E, Wean SE, Clendenon SS, Molitoris BA (2012) Multiple factors influence glomerular albumin permeability in rats. J Am Soc Nephrol 23(3):447–457

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Schiessl IM, Bardehle S, Castrop H (2013) Superficial nephrons in BALB/c and C57BL/6 mice facilitate in vivo multiphoton microscopy of the kidney. PLoS One 8(1):e52499

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Kalakeche R, Hato T, Rhodes G, Dunn KW, El-Achkar TM, Plotkin Z, Sandoval RM, Dagher PC (2011) Endotoxin uptake by S1 proximal tubular segment causes oxidative stress in the downstream S2 segment. J Am Soc Nephrol 22(8):1505–1516

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Phan TG, Bullen A (2010) Practical intravital two-photon microscopy for immunological research: faster, brighter, deeper. Immunol Cell Biol 88(4):438–444

    Article  PubMed  Google Scholar 

  13. Camirand G, Li Q, Demetris AJ, Watkins SC, Shlomchik WD, Rothstein DM, Lakkis FG (2011) Multiphoton intravital microscopy of the transplanted mouse kidney. Am J Transplant 11(10):2067–2074

    Article  CAS  PubMed  Google Scholar 

  14. Hato T, Winfree S, Kalakeche R, Dube S, Kumar R, Yoshimoto M, Plotkin Z, Dagher PC (2015) The macrophage mediates the renoprotective effects of endotoxin preconditioning. J Am Soc Nephrol 26(6):1347–1362

    Article  CAS  PubMed  Google Scholar 

  15. Berezin MY, Achilefu S (2010) Fluorescence lifetime measurements and biological imaging. Chem Rev 110(5):2641–2684

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Hato T, Friedman AN, Mang HE, Plotkin Z, Dube S, Hutchins GD, Territo PR, McCarthy BP, Riley AA, Pichumani K, Malloy CR, Harris RA, Dagher PC, Sutton TA (2016) Novel application of complimentary imaging techniques to examine in vivo glucose metabolism in the kidney. Am J Physiol Renal Physiol 310:F717–F727

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by National Institutes of Health (NIH) Grant R01 DK080067 (NIH), VA Merit (1I01BX002901-01A2), O’Brien Center grant P30DK079312 (NIH) to P.C.D. and Dialysis Clinics Inc. and Indiana Clinical and Translational Sciences Institute to T.H.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Pierre C. Dagher .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Hato, T., Winfree, S., Dagher, P.C. (2018). Kidney Imaging: Intravital Microscopy. In: Ishii, M. (eds) Intravital Imaging of Dynamic Bone and Immune Systems . Methods in Molecular Biology, vol 1763. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7762-8_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7762-8_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7761-1

  • Online ISBN: 978-1-4939-7762-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Navigation