Abstract
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a “closed-tube” system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or second sequence is typically adequate. The quantification process employs melting curve analysis and/or fluorescent detection systems and can provide amplification and genoty** in a relatively short time. Here we describe the properties and uses of various fluorescent detection systems used for quantification.
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Singh, C., Roy-Chowdhuri, S. (2016). Quantitative Real-Time PCR: Recent Advances. In: Luthra, R., Singh, R., Patel, K. (eds) Clinical Applications of PCR. Methods in Molecular Biology, vol 1392. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3360-0_15
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DOI: https://doi.org/10.1007/978-1-4939-3360-0_15
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