Abstract
Mass spectrometry-based phosphoproteomics has been rapidly spread based on the advancement of mass spectrometry and development of efficient enrichment techniques for phosphorylated proteins or peptides. Non-targeted approach has been employed in most of the studies for phosphoproteome analysis. However, targeted approach using selected/multiple reaction monitoring (SRM/MRM) is an indispensible technique used for the quantitation of known targets especially when we have many samples to quantitate phosphorylation events on proteins in biological or clinical samples. We herein describe the application of a large-scale phosphoproteome analysis and SRM-based quantitation for the systematic discovery and validation of biomarkers.
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Adachi, J., Narumi, R., Tomonaga, T. (2016). Targeted Phosphoproteome Analysis Using Selected/Multiple Reaction Monitoring (SRM/MRM). In: Reinders, J. (eds) Proteomics in Systems Biology. Methods in Molecular Biology, vol 1394. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3341-9_7
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DOI: https://doi.org/10.1007/978-1-4939-3341-9_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3339-6
Online ISBN: 978-1-4939-3341-9
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