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Systematic Analysis of Endocytosis by Cellular Perturbations

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Exocytosis and Endocytosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1174))

Abstract

Endocytosis is an essential process of eukaryotic cells that facilitates numerous cellular and organismal functions. The formation of vesicles from the plasma membrane serves the internalization of ligands and receptors and leads to their degradation or recycling. A number of distinct mechanisms have been described over the years, several of which are only partially characterized in terms of mechanism and function. These are often referred to as novel endocytic pathways. The pathways differ in their mode of uptake and in their intracellular destination. Here, an overview of the set of cellular proteins that facilitate the different pathways is provided. Further, the approaches to distinguish between the pathways by different modes of perturbation are critically discussed, emphasizing the use of genetic tools such as dominant negative mutant proteins.

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Acknowledgements

We would like to thank members of the Schelhaas laboratory for helpful comments and suggestions on the manuscript. Work in the Schelhaas laboratory is supported by the German Research Foundation (DFG) (grant SCHE 1552/2-1, Collaborative Research Centre SFB629/A16, International Graduate School GRK1409/C2, and partly by EXC 1003).

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  1. Emmy Noether Group: Virus Endocytosis, Institutes of Molecular Virology and Medical Biochemistry, ZMBE, Westphalian Wilhelms University of Münster, Von-Esmarch-Str. 56, Münster, 48149, Germany

    Lena Kühling & Mario Schelhaas

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  1. Lena Kühling
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  2. Mario Schelhaas
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Correspondence to Mario Schelhaas .

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  1. Department of Human and Molecular Genetics, VCU Institute of Molecular Medicine, Virginia Commonwealth University, Richmond, Virginia, USA

    Andrei I. Ivanov

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Kühling, L., Schelhaas, M. (2014). Systematic Analysis of Endocytosis by Cellular Perturbations. In: Ivanov, A. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 1174. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0944-5_2

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