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Multimodal Control of Bacterial Gene Expression by Red and Blue Light

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Synthetic Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2760))

Abstract

By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.

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Acknowledgements

Financial support was provided by the European Commission (FET Open NEUROPA, grant 863214 to A.M.), the Deutsche Forschungsgemeinschaft (grant MO2192/4-2 to A.M), the Academy of Finland (grant 330678 to H.T.), a three-year grant from the University of Helsinki (to E.M and H.T.), the Finnish Cultural Foundation (grant 00220697 to E.M.), and a Bayreuth Humboldt Centre Senior Fellowship 2020 (to H.T.).

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Authors and Affiliations

  1. Department of Biochemistry, University of Bayreuth, Bayreuth, Germany

    Stefanie S. M. Meier, Américo T. Ranzani & Andreas Möglich

  2. Department of Anatomy, University of Helsinki, Helsinki, Finland

    Elina Multamäki & Heikki Takala

  3. Department of Biological and Environmental Science, Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland

    Heikki Takala

  4. Bayreuth Center for Biochemistry & Molecular Biology, Universität Bayreuth, Bayreuth, Germany

    Andreas Möglich

  5. North-Bavarian NMR Center, Universität Bayreuth, Bayreuth, Germany

    Andreas Möglich

Authors
  1. Stefanie S. M. Meier
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  2. Elina Multamäki
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  3. Américo T. Ranzani
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  4. Heikki Takala
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  5. Andreas Möglich
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Corresponding author

Correspondence to Andreas Möglich .

Editor information

Editors and Affiliations

  1. Agilent Technologies, Inc, La Jolla, CA, USA

    Jeffrey Carl Braman

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Meier, S.S.M., Multamäki, E., Ranzani, A.T., Takala, H., Möglich, A. (2024). Multimodal Control of Bacterial Gene Expression by Red and Blue Light. In: Braman, J.C. (eds) Synthetic Biology. Methods in Molecular Biology, vol 2760. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3658-9_26

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  • DOI: https://doi.org/10.1007/978-1-0716-3658-9_26

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3657-2

  • Online ISBN: 978-1-0716-3658-9

  • eBook Packages: Springer Protocols

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