Abstract
Cas9-based genome editing is a powerful genetic tool for loci specifically targeted for genome modification. This chapter describes up-to-date protocols using Cas9-based genome editing technology, including vector construction with GoldenBraid assembly, Agrobacterium-mediated soybean transformation, and identification of editing in the genome.
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Quach, T., Nguyen, H., Meyer, O., Sato, S.J., Clemente, T.E., Guo, M. (2023). Introduction of Genome Editing Reagents and Genoty** of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.). In: Yang, B., Harwood, W., Que, Q. (eds) Plant Genome Engineering. Methods in Molecular Biology, vol 2653. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3131-7_17
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DOI: https://doi.org/10.1007/978-1-0716-3131-7_17
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3131-7
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