Abstract
Investigation of bovine nasal secretion has revealed a higher activity of alkaline phosphatase (ALP) than that found in serum. However it is not clear whether ALP is synthesized in the nasal tissue and the type of ALP isozyme that is produced in the secretion. The aim of this study was to measure the level of ALP activity and to separate the ALP isozymes in bovine nasal secretion and in extracts of relevant bovine tissues by isoelectric focusing (IEF).
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Reference
Ghazali, M.F., Jonsson, N.N., Burchmore, R.J.S. and Eckersall, P.D., 2012. Biochemical and proteomic investigation of bovine nasal secretion. Proceedings of the 3rd Managing Committee Meeting and 2nd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002 2:63–66.
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The Malaysian Ministry of Higher Education is thanked for funding MFG.
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Ghazali, M.F., Jonsson, N.N., McLaughlin, M., Macmillan, I., Eckersall, P.D. (2013). Isoelectric focusing in characterisation of alkaline phosphatase isozyme from bovine nasal secretion and mucosa. In: de Almeida, A., et al. Farm animal proteomics 2013. Wageningen Academic Publishers, Wageningen. https://doi.org/10.3920/978-90-8686-776-9_26
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DOI: https://doi.org/10.3920/978-90-8686-776-9_26
Publisher Name: Wageningen Academic Publishers, Wageningen
Online ISBN: 978-90-8686-776-9
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