Abstract
The potential applications of acyltransferases in the synthesis of pharmaceutically significant hydroxamic acids have rendered it substantial industrial importance. Enrichment techniques were used to isolate a thermophilic bacterial strain which is capable of degrading acetamide. This isolate was classified as Bacillus sp. on the basis of morphology, biochemical and physiological tests. Based on the 16S ribosomal RNA sequencing, the isolate was designated as Bacillus megaterium. Its resting cells containing the active acyltransferase enzyme were immobilized in the gel beads of different matrices as sodium alginate, agar, polyacrylamide and polyvinyl alcohol-alginate. The assay of acyltransferase activity of the immobilized beads was done using iron (III) chloride reagents at high temperature of 55 °C. The best performing sodium alginate beads were optimized for various parameters such as substrate concentration, temperature, pH and the type of buffer. This chapter provides insights on acyltransferase enzyme immobilization technique, its operational stability and its significance in hydroxamic acid synthesis.
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Syed, Z., Sogani, M. (2022). Emerging Technique of Enzymatic Biotransformation of Amides to Hydroxamic Acid for Pharmaceutical and Dye Waste Treatment. In: Arora, S., Kumar, A., Ogita, S., Yau, Y.Y. (eds) Innovations in Environmental Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-16-4445-0_30
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