SpringerLink
Log in

The Utility of DNA Barcoding Technology in the Authentication of Medicinal Plants in Illegal Trade: A Critical Review

  • Chapter
  • First Online:
Forensic DNA Ty**: Principles, Applications and Advancements

Abstract

DNA barcoding technology is the utilization of short segments of DNA for the rapid and accurate identification of closely related species. The technology offers advantages over other taxonomic methods of identification, as it requires very minute tissue specimen. DNA barcoding is a project with mission to provide unique genomic database for the identification of all types of eukaryotes. The utilization of barcode has helped so far to discover new species which were once considered merged with species having similar morphology, but now with the evolution of genetic DNA barcoding sequences, organisms can be differentiated from the closely related species as well as phylogenetic relationships can be discovered. The technique holds potential to resolve fundamental ecological, evolutionary, biological, herbal authentication disputes, as well as answer to unresolved forensic questions. In this chapter, the use of DNA barcoding in wildlife forensics and its application in forensic science are discussed. Various aspects of DNA barcoding technology are highlighted specifically with respect to illegal trade of medicinal plants; identification and authentication of medicinal plants with detailed protocols for extraction, and amplification and sequencing of DNA barcoding are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (Brazil)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (Brazil)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (Brazil)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
USD 279.99
Price excludes VAT (Brazil)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Al-Qurainy F, Khan S, Ali MA, Al-Hemaid FM, Tarroum M, Ashraf M (2011) Authentication of Ruta graveolens and ITS adulterant using internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Pak J Bot 43(3):1613–1620

    CAS  Google Scholar 

  • Aubriot X, Lowry PP, Cruaud C, Couloux A, Haevermans T (2013) DNA barcoding in a biodiversity hot spot: potential value for the identification of Malagasy Euphorbia L. listed in CITES Appendices I and II. Mol Ecol Resour 13(1):57–65

    Article  CAS  PubMed  Google Scholar 

  • Bolson M, de Camargo Smidt E, Brotto ML, Silva-Pereira V (2015) ITS and trnH-psbA as efficient DNA barcodes to identify threatened commercial woody angiosperms from southern Brazilian Atlantic rainforests. PLoS One 10(12):e0143049

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Bordoloi R, Dutta KN (2014) A review on herbs used in the treatment of diabetes mellitus. J Pharm Chem Biol Sci 2:86–92

    CAS  Google Scholar 

  • Bruyns PV, Mapaya RJ, Hedderson TJ (2006) A new subgeneric classification for Euphorbia (Euphorbiaceae) in southern Africa based on ITS and psbA-trnH sequence data. Taxon 55(2):397–420

    Article  Google Scholar 

  • Burgess KS, Fazekas AJ, Kesanakurti PR, Graham SW, Husband BC, Newmaster SG, Percy DM, Hajibabaei M, Barrett SC (2011) Discriminating plant species in a local temperate flora using the rbcL+ matK DNA barcode. Methods Ecol Evol 2(4):333–340

    Article  Google Scholar 

  • Butchart SH, Stattersfield AJ, Bennun LA, Shutes SM, Akçakaya HR, Baillie JE et al (2004) Measuring global trends in the status of biodiversity: red list indices for birds. PLoS Biol 2(12):e383

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Carvalho DC, Palhares RM, Drummond MG, Frigo TB (2015) DNA barcoding identification of commercialized seafood in South Brazil: a governmental regulatory forensic program. Food Control 50:784–788

    Article  CAS  Google Scholar 

  • Chandramohan A, Divya SR, Dhanarajan MS (2013) MatK gene based molecular characterization of medicinal plant—Croton bonplandianum Baill. Int J Biosci Res 2:1–7

    Google Scholar 

  • Chen S, Yao H, Han J, Liu C, Song J, Shi L, Zhu Y, Ma X, Gao T, Pang X, Luo K (2010) Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One 5(1):e8613

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Cheng T, Xu C, Lei L, Li C, Zhang Y, Zhou S (2016) Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity. Mol Ecol Resour 16(1):138–149

    Article  CAS  PubMed  Google Scholar 

  • Coghlan ML, Haile J, Houston J, Murray DC, White NE, Moolhuijzen P et al (2012) Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and health safety concerns. PLoS Genet 8(4):e1002657

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cooper JE, Cooper ME (2013) Wildlife forensic investigation: principles and practice. CRC Press, Boca Raton

    Book  Google Scholar 

  • Cowan RS, Fay MF (2012) Challenges in the DNA barcoding of plant material. In: Plant DNA fingerprinting and barcoding. Humana Press, Totowa, NJ, pp 23–33

    Chapter  Google Scholar 

  • Cuénoud P, Savolainen V, Chatrou LW, Powell M, Grayer RJ, Chase MW (2002) Molecular phylogenetics of Caryophyllales based on nuclear 18S rDNA and plastid rbcL, atpB, and matK DNA sequences. Am J Bot 89(1):132–144

    Article  PubMed  Google Scholar 

  • Dawson IK, Powell W (1999) Genetic variation in the Afromontane tree Prunus africana, an endangered medicinal species. Mol Ecol 8(1):151–156

    Article  Google Scholar 

  • Dey SC (n.d.) The convention on international trade in endangered species of wild fauna and flora (CITES)

    Google Scholar 

  • Douwma G, Hollow T (2014) TRAFFIC’s vision. TRAFFIC Bull 26(1)

    Google Scholar 

  • Dunning LT, Savolainen V (2010) Broad-scale amplification of matK for DNA barcoding plants, a technical note. Bot J Linn Soc 164(1):1–9

    Article  Google Scholar 

  • Enan MR, Ahamed A (2014) DNA barcoding based on plastid matK and RNA polymerase for assessing the genetic identity of date (Phoenix dactylifera L.) cultivars. Genet Mol Res 13(2):3527–3536

    Article  CAS  PubMed  Google Scholar 

  • Fazekas AJ, Burgess KS, Kesanakurti PR, Graham SW, Newmaster SG, Husband BC, Percy DM, Hajibabaei M, Barrett SC (2008) Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. PLoS One 3(7):e2802

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Fazekas AJ, Kesanakurti PR, Burgess KS, Percy DM, Graham SW, Barrett SC, Newmaster SG, Hajibabaei M, Husband BC (2009) Are plant species inherently harder to discriminate than animal species using DNA barcoding markers? Mol Ecol Resour 9:130–139

    Article  CAS  PubMed  Google Scholar 

  • Fazekas AJ, Kuzmina ML, Newmaster SG, Hollingsworth PM (2012) DNA barcoding methods for land plants. In: DNA barcodes. Humana Press, Totowa, NJ, pp 223–252

    Chapter  Google Scholar 

  • Ferri G, Corradini B, Ferrari F, Santunione AL, Palazzoli F, Alu M (2015) Forensic botany II, DNA barcode for land plants: which markers after the international agreement? Forensic Sci Int Genet 15:131–136

    Article  CAS  PubMed  Google Scholar 

  • Gao T, Yao H, Song J, Zhu Y, Liu C, Chen S (2010) Evaluating the feasibility of using candidate DNA barcodes in discriminating species of the large Asteraceae family. BMC Evol Biol 10(1):324

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gernandt DS, López GG, García SO, Liston A (2005) Phylogeny and classification of Pinus. Taxon 54(1):29–42

    Article  Google Scholar 

  • Group CPW, Hollingsworth PM, Forrest LL, Spouge JL, Hajibabaei M, Ratnasingham S, van der Bank M, Chase MW, Cowan RS, Erickson DL, Fazekas AJ (2009) A DNA barcode for land plants. Proc Natl Acad Sci U S A 106(31):12794–12797

    Article  Google Scholar 

  • Group CPB, Li DZ, Gao LM, Li HT, Wang H, Ge XJ, Liu JQ, Chen ZD, Zhou SL, Chen SL, Yang JB (2011) Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. Proc Natl Acad Sci U S A 108(49):19641–19646

    Article  Google Scholar 

  • Guo X, Wang X, Su W, Zhang G, Zhou R (2011) DNA barcodes for discriminating the medicinal plant Scutellaria baicalensis (Lamiaceae) and its adulterants. Biol Pharm Bull 34(8):1198–1203

    Article  CAS  PubMed  Google Scholar 

  • Haibin W, Kunming G (1999) Wildlife trade in Yunnan Province, China, at the border with Vietnam. TRAFFIC Bull 18:21–30

    Google Scholar 

  • Hajibabaei M, deWaard JR, Ivanova NV, Ratnasingham S, Dooh RT, Kirk SL, Mackie PM, Hebert PD (2005) Critical factors for assembling a high volume of DNA barcodes. Philos Trans R Soc B Biol Sci 360(1462):1959–1967

    Article  CAS  Google Scholar 

  • Hajibabaei M, Smith MA, Janzen DH, Rodriguez JJ, Whitfield JB, Hebert PD (2006) A minimalist barcode can identify a specimen whose DNA is degraded. Mol Ecol Notes 6(4):959–964

    Article  CAS  Google Scholar 

  • Hajibabaei M, Singer GA, Hebert PD, Hickey DA (2007) DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trends Genet 23(4):167–172

    Article  CAS  PubMed  Google Scholar 

  • Halward TM, Stalker HT, Larue EA, Kochert G (1991) Genetic variation detectable with molecular markers among unadapted germ-plasm resources of cultivated peanut and related wild species. Genome 34(6):1013–1020

    Article  CAS  Google Scholar 

  • Han J, Zhu Y, Chen X, Liao B, Yao H, Song J, Chen S, Meng F (2013) The short ITS2 sequence serves as an efficient taxonomic sequence tag in comparison with the full-length ITS. BioMed Res Int 2013:741476

    PubMed  PubMed Central  Google Scholar 

  • Hasebe M, Omori T, Nakazawa M, Sano T, Kato M, Iwatsuki K (1994) rbcL gene sequences provide evidence for the evolutionary lineages of leptosporangiate ferns. Proc Natl Acad Sci U S A 91(12):5730–5734

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hebert PD, Cywinska A, Ball SL, Dewaard JR (2003) Biological identifications through DNA barcodes. Proc R Soc Lond Ser B Biol Sci 270(1512):313–321

    Article  CAS  Google Scholar 

  • Hollingsworth PM (2008) DNA barcoding plants in biodiversity hot spots: progress and outstanding questions. Heredity (Edinb) 101(1):1–2

    Article  CAS  Google Scholar 

  • Hollingsworth PM, Graham SW, Little DP (2011) Choosing and using a plant DNA barcode. PLoS One 6(5):e19254

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hou D, Song J, Shi L, Ma X, **n T, Han J, **ao W, Sun Z, Cheng R, Yao H (2013) Stability and accuracy assessment of identification of traditional Chinese materia medica using DNA barcoding: a case study on Flos Lonicerae Japonicae. BioMed Res Int 2013(1):549037

    PubMed  PubMed Central  Google Scholar 

  • Hu Z, Tu Y, **a Y, Cheng P, Sun W, Shi Y, Guo L, He H, **ong C, Chen S, Zhang X (2015) Rapid identification and verification of indirubin-containing medicinal plants. Evid Based Complementary Altern Med 2015:484670

    Google Scholar 

  • Krawczyk K, SzczeciÅ„ska M, Sawicki J (2014) Evaluation of 11 single-locus and seven multilocus DNA barcodes in Lamium L. (Lamiaceae). Mol Ecol Resour 14(2):272–285

    Article  CAS  PubMed  Google Scholar 

  • Kress WJ, Erickson DL (2007) A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS One 2(6):e508

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kress WJ, García-Robledo C, Uriarte M, Erickson DL (2015) DNA barcodes for ecology, evolution, and conservation. Trends Ecol Evol 30(1):25–35

    Article  PubMed  Google Scholar 

  • Lahaye R, Van der Bank M, Bogarin D, Warner J, Pupulin F, Gigot G, Maurin O, Duthoit S, Barraclough TG, Savolainen V (2008) DNA barcoding the floras of biodiversity hotspots. Proc Natl Acad Sci U S A 105(8):2923–2928

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lange DAGMAR (2006) International trade in medicinal and aromatic plants: actors, volumes and commodities. Frontis:155–170

    Google Scholar 

  • Lee SS, Jeong WJ, Bae JM, Bang JW, Liu JR, Harn CH (2004) Characterization of the plastid-encoded carboxyltransferase subunit (accD) gene of potato. Mol Cells 17(3):422–429

    CAS  PubMed  Google Scholar 

  • Lee SC, Chiou SJ, Yen JH, Lin TY, Hsieh KT, Yang JC (2010) DNA barcoding Cinnamomum osmophloeum Kaneh. Based on the partial non-coding ITS2 region of ribosomal genes. J Food Drug Anal 18(2)

    Google Scholar 

  • Li Y, Ruan J, Chen S, Song J, Luo K, Lu D, Yao H (2010) Authentication of Taxillus chinensis using DNA barcoding technique. J Med Plants Res 4(24):2706–2709

    Article  CAS  Google Scholar 

  • Li FW, Kuo LY, Rothfels CJ, Ebihara A, Chiou WL, Windham MD, Pryer KM (2011) rbcL and matK earn two thumbs up as the core DNA barcode for ferns. PLoS One 6(10):e26597

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li X, Yang Y, Henry RJ, Rossetto M, Wang Y, Chen S (2015) Plant DNA barcoding: from gene to genome. Biol Rev 90(1):157–166

    Article  PubMed  Google Scholar 

  • Linacre A, Tobe SS (2011) An overview to the investigative approach to species testing in wildlife forensic science. Investig Genet 2(1):2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Little DP (2014) Authentication of Ginkgo biloba herbal dietary supplements using DNA barcoding. Genome 57(9):513–516

    Article  CAS  PubMed  Google Scholar 

  • Lledo MD, Crespo MB, Cameron KM, Fay MF, Chase MW (1998) Systematics of Plumbaginaceae based upon cladistic analysis of rbcL sequence data. Syst Bot 23:21–29

    Article  Google Scholar 

  • Ma XY, **e CX, Liu C, Song JY, Yao H, Luo K, Zhu YJ, Gao T, Pang XH, Qian J, Chen SL (2010) Species identification of medicinal pteridophytes by a DNA barcode marker, the chloroplast psbA-trnH intergenic region. Biol Pharm Bull 33(11):1919–1924

    Article  CAS  PubMed  Google Scholar 

  • Mishra P, Kumar A, Nagireddy A, Mani DN, Shukla AK, Tiwari R, Sundaresan V (2016) DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market. Plant Biotechnol J 14(1):8–21

    Article  CAS  PubMed  Google Scholar 

  • Muellner AN, Schaefer H, Lahaye R (2011) Evaluation of candidate DNA barcoding loci for economically important timber species of the mahogany family (Meliaceae). Mol Ecol Resour 11(3):450–460

    Article  CAS  PubMed  Google Scholar 

  • Newmaster SG, Fazekas AJ, Ragupathy S (2006) DNA barcoding in land plants: evaluation of rbcL in a multigene tiered approach. Botany 84(3):335–341

    CAS  Google Scholar 

  • Newmaster SG, Grguric M, Shanmughanandhan D, Ramalingam S, Ragupathy S (2013) DNA barcoding detects contamination and substitution in North American herbal products. BMC Med 11(1):222

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Nithaniyal S, Newmaster SG, Ragupathy S, Krishnamoorthy D, Vassou SL, Parani M (2014) DNA barcode authentication of wood samples of threatened and commercial timber trees within the tropical dry evergreen forest of India. PLoS One 9(9):e107669

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Palhares RM, Drummond MG, Brasil BDSAF, Cosenza GP, Brandão MDGL, Oliveira G (2015) Medicinal plants recommended by the world health organization: DNA barcode identification associated with chemical analyses guarantees their quality. PLoS One 10(5):e0127866

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Pan S-Y, Litscher G, Gao S-H, Zhou S-F, Yu Z-L, Chen H-Q, Zhang S-F, Tang M-K, Sun J-N, Ko K-M (2014) Historical perspective of traditional indigenous medical practices: the current renaissance and conservation of herbal resources. Evid Based Complement Alternat Med 2014:1

    Google Scholar 

  • Pang X, Song J, Zhu Y, **e C, Chen S (2010) Using DNA barcoding to identify species within Euphorbiaceae. Planta Med 76(15):1784–1786

    Article  CAS  PubMed  Google Scholar 

  • Parvathy VA, Swetha VP, Sheeja TE, Leela NK, Chempakam B, Sasikumar B (2014) DNA barcoding to detect chilli adulteration in traded black pepper powder. Food Biotechnol 28(1):25–40

    Article  CAS  Google Scholar 

  • Pawlowski J, Audic S, Adl S, Bass D, Belbahri L, Berney C, Bowser SS, Cepicka I, Decelle J, Dunthorn M, Fiore-Donno AM (2012) CBOL protist working group: barcoding eukaryotic richness beyond the animal, plant, and fungal kingdoms. PLoS Biol 10(11):e1001419

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rajendran R, Basha NS (2010) Antimicrobial activity of crude extracts and fractions of Premna serratifolia Linn., root. Med Plants 2(1):1–6

    Google Scholar 

  • Reeve R (2014) Policing international trade in endangered species: the CITES treaty and compliance. Routledge, UK

    Book  Google Scholar 

  • Rogers SO, Bendich AJ (1989) Extraction of DNA from plant tissues. In: Plant molecular biology manual. Springer, Dordrecht, pp 73–83

    Chapter  Google Scholar 

  • Saarela JM, Sokoloff PC, Gillespie LJ, Consaul LL, Bull RD (2013) DNA barcoding the Canadian Arctic flora: core plastid barcodes (rbcL+ matK) for 490 vascular plant species. PLoS One 8(10):e77982

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sass C, Little DP, Stevenson DW, Specht CD (2007) DNA barcoding in the cycadales: testing the potential of proposed barcoding markers for species identification of cycads. PLoS One 2(11):e1154

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Schori M, Showalter AM (2011) DNA barcoding as a means for identifying medicinal plants of Pakistan. Pak J Bot 43:1–4

    CAS  Google Scholar 

  • Sen S, Chakraborty R, De B (2011) Challenges and opportunities in the advancement of herbal medicine: India’s position and role in a global context. J Herb Med 1(3–4):67–75

    Article  Google Scholar 

  • Shokralla S, Spall JL, Gibson JF, Hajibabaei M (2012) Next-generation sequencing technologies for environmental DNA research. Mol Ecol 21(8):1794–1805

    Article  CAS  PubMed  Google Scholar 

  • Subrat N, Iyer M, Prasad R (2002) The ayurvedic medicine industry: current status and sustainability. Ecotech Services, London, and International Institute for Environment and Development, New Delhi

    Google Scholar 

  • Techen N, Parveen I, Pan Z, Khan IA (2014) DNA barcoding of medicinal plant material for identification. Curr Opin Biotechnol 25:103–110

    Article  CAS  PubMed  Google Scholar 

  • Theodoridis S, Stefanaki A, Tezcan M, Aki C, Kokkini S, Vlachonasios KE (2012) DNA barcoding in native plants of the Labiatae (Lamiaceae) family from Chios Island (Greece) and the adjacent ÇeÅŸme-Karaburun Peninsula (Turkey). Mol Ecol Resour 12(4):620–633

    Article  PubMed  Google Scholar 

  • Valentini A, Pompanon F, Taberlet P (2009) DNA barcoding for ecologists. Trends Ecol Evol 24(2):110–117

    Article  PubMed  Google Scholar 

  • Vassou SL, Kusuma G, Parani M (2015) DNA barcoding for species identification from dried and powdered plant parts: a case study with authentication of the raw drug market samples of Sida cordifolia. Gene 559(1):86–93

    Article  CAS  PubMed  Google Scholar 

  • Vidyarthi S, Samant SS, Sharma P (2013) Dwindling status of Trillium govanianum Wall. ex D. Don-A case study from Kullu district of Himachal Pradesh, India. J Med Plants Res 7(8):392–397

    Google Scholar 

  • Vijayan K, Tsou CH (2010) DNA barcoding in plants: taxonomy in a new perspective. Curr Sci 99:1530–1541

    CAS  Google Scholar 

  • Wakdikar S (2004) Global health care challenge: Indian experiences and new prescriptions. Electron J Biotechnol 7(3):02–03

    Article  Google Scholar 

  • Warchol GL (2004) The transnational illegal wildlife trade. Crim Justice Stud 17(1):57–73

    Article  Google Scholar 

  • Wasser SK, Joseph Clark WILLIAM, Drori O, Stephen Kisamo EMILY, Mailand C, Mutayoba B, Stephens M (2008) Combating the illegal trade in African elephant ivory with DNA forensics. Conserv Biol 22(4):1065–1071

    Article  PubMed  Google Scholar 

  • Wellsmith M (2011) Wildlife crime: the problems of enforcement. Eur J Crim Policy Res 17(2):125–148

    Article  Google Scholar 

  • Werle E, Schneider C, Renner M, Völker M, Fiehn W (1994) Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Res 22(20):4354

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wong EHK, Hanner RH (2008) DNA barcoding detects market substitution in North American seafood. Food Res Int 41(8):828–837

    Article  CAS  Google Scholar 

  • Wong KL, But PH, Shaw PC (2013) Evaluation of seven DNA barcodes for differentiating closely related medicinal Gentiana species and their adulterants. Chin Med 8(1):16

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Wyatt T, Johnson K, Hunter L, George R, Gunter R (2018) Corruption and wildlife trafficking: three case studies involving Asia. Asian J Criminol 13(1):35–55

    Article  Google Scholar 

  • Yao H, Song JY, Ma XY, Liu C, Li Y, Xu HX, Han JP, Duan LS, Chen SL (2009) Identification of Dendrobium species by a candidate DNA barcode sequence: the chloroplast psbA-trnH intergenic region. Planta Med 75(06):667–669

    Article  CAS  PubMed  Google Scholar 

  • Yao H, Song J, Liu C, Luo K, Han J, Li Y, Pang X, Xu H, Zhu Y, **ao P, Chen S (2010) Use of ITS2 region as the universal DNA barcode for plants and animals. PLoS One 5(10):e13102

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Yu J, Xue JH, Zhou SL (2011) New universal matK primers for DNA barcoding angiosperms. J Syst Evol 49(3):176–181

    Article  Google Scholar 

  • Zhou J, Wang W, Liu M, Liu Z (2014) Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by DNA-barcoding technique. Pharmacogn Mag 10(40):385

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Forensic Science, Punjabi University, Patiala, Punjab, India

    Mukesh Kumar Thakar

  2. Department of Forensic Science and Toxicology, Chandigarh University, Mohali, Punjab, India

    Tina Sharma

Authors
  1. Mukesh Kumar Thakar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tina Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. DNA Fingerprinting Unit, Department of Home (Police), State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Pankaj Shrivastava

  2. DNA Fingerprinting Unit, Regional Forensic Science Laboratory, Bhopal, Madhya Pradesh, India

    Hirak Ranjan Dash

  3. Department of Legal Medicine, University of Granada, Granada, Granada, Spain

    Jose A. Lorente

  4. DNA Fingerprinting Unit, State Forensic Science Laboratory, Ranchi, Jharkhand, India

    Jahangir Imam

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Thakar, M.K., Sharma, T. (2020). The Utility of DNA Barcoding Technology in the Authentication of Medicinal Plants in Illegal Trade: A Critical Review. In: Shrivastava, P., Dash, H.R., Lorente, J.A., Imam, J. (eds) Forensic DNA Ty**: Principles, Applications and Advancements . Springer, Singapore. https://doi.org/10.1007/978-981-15-6655-4_21

Download citation

Publish with us

Policies and ethics

Search

Navigation