Abstract
In this study, Xylose-Taurine reduced (X-T-R) was synthesized to enhance biological activities. Hence, we investigated the hepatoprotective effects of X-T-R against H2O2-induced hepatocyte damage and apoptosis. The results showed that X-T-R led to the cytoprotective effect against H2O2-induced oxidative stress in cultured hepatocytes such as the improvement of cell viability and the reduction of reactive oxygen species (ROS) production. Additionally, pre-treatment with X-T-R increased the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H dehydrogenase:quinone 1 (NQO1) and heme oxygenase 1 (HO-1) in cultured hepatocytes. Furthermore, X-T-R protected the cells against apoptosis via regulating the expression level of Bcl-2/Bax as well as the activation of caspase-3. According to the results obtained, X-T-R may be a bio-material for the therapy of hepatic diseases.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
Abbreviations
- HO-1:
-
Heme oxygenase 1
- NQO1:
-
NAD(P)H dehydrogenase:quinone
- Nrf2:
-
Nuclear factor erythroid 2-related factor 2
- X-T-R:
-
Xylose-Taurine reduced
References
Adachi M, Higuchi H, Miura S, Azuma T, Inokuchi S, Saito H, Kato S, Ishii H (2004) Bax interacts with the voltage-dependent anion channel and mediates ethanol-induced apoptosis in rat hepatocytes. Am J Phys 287:695–705
Auroma OI (1994) Nutrition and health aspect of free radicals and antioxidants. Food Chem Toxicol 32:671–683
Campbell MR, Karaca M, Adamski KN, Chorley BN, Wang X, Bell DA (2013) Novel hematopoietic target genes in the NRF2-mediated transcriptional pathway. Oxid Med Cell Logev 2013:120305
Chiu YW, Lo HJ, Huang HY, Chao PY, Huang JM, Huang PY, Huang SJ, Liu JY, Lai TJ (2013) The antioxidant and cytoprotective activity of Ocimum gratissimum extracts against hydrogen peroxide-induced toxicity in human HepG2 cells. J Food Drug Anal 21:253–260
Cho HJ, You JS, Chang KJ, Kim KS, Kim SH (2014) Anti-adipogenic effect of taurine-carbohydrate derivatives. Bull Kor Chem Soc 35:1863–1866
Engelmann J, Volk J, Leyhausen G, Geurtsen W (2005) ROS formation and glutathione levels in human oral fibroblasts exposed to TEGDMA and camphorquinone. J Biomed Mat Res B 75(2):272–276
Ghosh M, Manna P, Sil PC (2011) Protective role of a coumarin-derived schiff base scaffold against tertiary butyl hydroperoxide (TBHP)-induced oxidative impairment and cell death via MAPKs, NF-kB and mitochondria-dependent pathways. Free Radic Res 45:620–637
Kang MC, Kim KN, Kang SM, Yang X, Kim EA, Song CB, Nah JW, Jang MK, Lee JS, Jung WK, Jeon YJ (2013) Protective effect of dieckol isolated from Ecklonia cava against ethanol caused damage in vitro and in zebrafish model. Environ Toxicol Pharmacol 36:1217–1226
Kay HY, Won YJ, Kim TH, Lee DY, Kang B, Ryu JH, Jeon R, Kim SG (2010) Ajoene, a stable garlic by-product, has an antioxidant effect through Nrf2-mediated glutamate-cysteine ligase induction in HepG2 cells and primary hepatocytes. J Nutr 140:1211–1219
Kim KC, Kang KA, Zhang R, Piao MJ, Kim GY, Kang MY, Lee SY, Lee NH, Surh HJ, Hyun JW (2010) Up-regulation of Nrf2-mediated heme oxygenase-1 expression by eckol, a phlorotannin compound, though activation of Erk and PI3K/Akt. Int J Biochem Cell Biol 42:297–305
Kumar H, Lim HW, More SV, Kim BW, Koppula S, Kim IS, Choi DK (2012) The role of free radicals in the aging brain and Parkinson’s disease: convergence and parallelism. Int J Mol Sci 13:10478–10504
Leiser SF, Miller RA (2010) Nrf2 signaling a mechanism for cellular stress resistance in long-lived mice. Mol Cell Biol 30:871–884
Li M, Lu Y, Hu Y, Zhai X, Xu W, **g H, Tian X, Lin Y, Gao D, Yao J (2014) Salvianolic acid B protects against acute ethanol-induced liver injury through SIRT1-mediated deacetylation of p53 in rats. Toxicol Lett 228:67–74
Masella R, Di BR, Vari R, Filesi C, Giovannini C (2005) Novel mechanisms of natural antioxidant compounfs in biological systems: involvement of glutathione-related enzymes. J Nutr Biochem 16:577–586
Nakagawa K, Huxtable RJ (1985) The anticonvulsive actions of two lipophilic taurine derivatives. Neurochem Int 7:819–824
Nioi P, Hayes JD (2004) Contribution of NAD(P)H:quinone oxidoreductase 1 to protection against carcinogenesis, and regulation of its gene by Nrf2 basic-region leucine zipper and the arylhydrocarbon receptor basic helix-loop-helix transcription factors. Mutat Res 555:149–171
Niture SK, Kaspar JW, Shen J, Jaiswal AK (2010) Nrf2 signaling and cell survival. Toxicol Appl Pharmacol 244:37–42
Okezie IA (1998) Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc 75:199–212
Renehan AG, Booth C, Potten CS (2001) What is apoptosis, and why is it important? BMJ 322:1536–1538
Rigoulet M, Yoboue ED, Devin A (2011) Mitochondrial ROS generation and its regulation: mechanisms involved in H2O2 signaling. Antioxid Redox Signal 14:459–468
Ryter SW, Otterbein LE, Morse D, Choi AM (2002) Heme oxygenase/carbon monoxide signaling pathways: regulation and functional significance. Mol Cell Biochem 234–235:249–263
Surh YJ, Kundu JK, Li MH, Na HK, Cha YN (2009) Role of Nrf2-mediated heme oxygenase-1 upregulation in adaptive survival response to nitrosative stress. Arch Pharm Res 32:1163–1176
Trachootham D, Lu W, Ogasawara MA, Nilsa RD, Huang P (2008) Redox regulation of cell survival. Antioxid Redox Signal 10:1343–1374
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84
Xu D, Hu L, **a X, Song J, Li L, Song E, Song Y (2014) Tetrachlorobenzoquinone induces acute liver injury, up-regulates HO-1 and NQO1 expression in mice model: the protective role of chlorogenic acid. Environ Toxicol Pharmacol 37:1212–1220
Yahn SL, Watterson LR, Olive MF (2013) Safety and efficacy of acamprosate for the treatment of alcohol dependence. Subst Abus 6:1–12
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media B.V.
About this paper
Cite this paper
Park, S.Y. et al. (2017). Hepatoprotective Effects of Xylose-Taurine Reduced Against Hydrogen Peroxide-Induced Oxidative Stress in Cultured Hepatocytes. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_48
Download citation
DOI: https://doi.org/10.1007/978-94-024-1079-2_48
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-024-1077-8
Online ISBN: 978-94-024-1079-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)