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Protective Effects of An Enzymatic Hydrolysate from Octopus ocellatus Meat against Hydrogen Peroxide-Induced Oxidative Stress in Chang Liver Cells and Zebrafish Embryo

  • Conference paper
Taurine 10

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 975))

Abstract

Octopus ocellatus, a marine cephalopod distributed in the coast of South Korea, China, Japan and tropical sea, contains high amounts of taurine. In this study, an enzymatic hydrolysate obtained from O. ocellatus meat was evaluated for its antioxidant effects using a human liver cell line and zebrafish embryo model. Enzymatic hydrolysates of the O. ocellatus meat (OOM) were prepared using six different enzymes. Among the enzymatic hydrolysates, Alcalase hydrolysate of OOM (OOMAH) showed the highest scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals and hydrogen peroxide (H2O2). Moreover, it showed a high oxygen radical absorbance capacity (ORAC). OOMAH treatment effectively reduced the hydroxyl radical-induced DNA damage. OOMAH reduced the production of reactive oxygen species (ROS) in H2O2-treated hepatocytes without cytotoxicity. Furthermore, OOMAH improved the survival rate and reduced the intracellular ROS levels in H2O2-treated zebrafish embryos. Compositional analysis of amino acids indicated a high content of taurine in OOMAH. Current results suggest that OOMAH possesses antioxidant bioactivities and could provide protective effects against H2O2-induced oxidative stress. Therefore, OOMAH might be used as a potential resource of functional foods.

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Abbreviations

ORAC:

oxygen radical absorbance capacity

OOM:

Octopus ocellatus meat

OOMAH:

Alcalase hydrolysate of O. ocellatus meat

ROS:

Reactive oxygen species

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Author information

Author notes

  1. $S.H. Lee and G. Ahn contributed equally to this study.

Authors and Affiliations

  1. Department of Food Technology and Nutrition, Chonnam National University, Yeosu, South Korea

    Ju Hyung Um, Eui Jeong Han, Soo Yeon Park & Ginnae Ahn$

  2. Department of Marine Life Science, Jeju National University, Jeju, South Korea

    Eun-A Kim, WonWoo Lee, Nalae Kang & Jae Young Oh

  3. Division of Food Bioscience and Korea Nokyong Research Center, Konkuk University, Chungju, South Korea

    You-** Jeon & Seung-Hong Lee

  4. Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, South Korea

    Ginnae Ahn$

Authors
  1. Ju Hyung Um
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  2. Eun-A Kim
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  3. WonWoo Lee
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  4. Nalae Kang
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  5. Eui Jeong Han
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  7. Soo Yeon Park
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  9. Seung-Hong Lee
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  10. Ginnae Ahn$
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Corresponding author

Correspondence to Ginnae Ahn$ .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Seoul, Seoul, South Korea

    Dong-Hee Lee  & Ha Won Kim  & 

  2. College of Medicine, University of South Alabama, Mobile, Alabama, USA

    Stephen W. Schaffer

  3. Department of Developmental Neurobiology, NY State Institute for Basic Research in Developmental Disabilities, New York, USA

    Eunkyue Park

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Um, J.H. et al. (2017). Protective Effects of An Enzymatic Hydrolysate from Octopus ocellatus Meat against Hydrogen Peroxide-Induced Oxidative Stress in Chang Liver Cells and Zebrafish Embryo. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_47

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