Applications of LC-MS in Plant Metabolomics

  • Chapter
  • First Online:
Plant Metabolomics
  • 1859 Accesses

Abstract

Although plant metabolomics is still in the development stage, it is develo** rapidly. Current international working is on the large-scale metabolomics data accumulation, namely the use of a variety of chemical analytical platforms, especially mass-based GC-MS and LC-MS in plant (mostly concentrate on the model plant Arabidopsis, including different mutants, ecotypes, tissues, and treatments with different conditions) metabolomics analysis. The international plant metabolomics society established a set of standardized procedures on different plant metabolomics analytical tools, including experimental design, data processing, and reporting, and making the metabolomics data sharing between different laboratories is possible (Feighn in Metabolomics 3:175–178, 2007), which will greatly improve the role of metabolomics in plant research. Plant metabolomics also evolved from simple metabolic analysis to systematically reveal the mechanism of plant cell by combining with other omics techniques.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Berrueta LA, Gallo B, Vicente F. A review of solid phase extraction: basic principles and new developments. Chromatographia. 1995;40:474–83.

    Article  CAS  Google Scholar 

  • Catchpole GS, Beckmann M, Enot DP, Mondhe M, Zywicki B, Taylor J, Hardy N, Smith A, King RD, Kell DB, Fiehn O, Draper J. Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato crops. Proc Natl Acad Sci USA. 2005;102:14458–14462.

    Google Scholar 

  • Fiehn O, Robertson D, Griffin J, van der Werf M, Nikolau B, Morrison N, Sumner LW, Goodacre R, Hardy NW, Taylor C, Fostel J, Kristal B, Kaddurah-Daouk R, Mendes P, van Ommen B, Lindon JC, Sansone SA. The metabolomics standards initiative (MSI). Metabolomics. 2007;3:175–8.

    Article  CAS  Google Scholar 

  • Fukushima A, Kusano M, Redestig H, Arita M, Saito K. Integrated omics approaches in plant systems biology. Curr Opin Chem Biol. 2009;13:532–8.

    Article  CAS  PubMed  Google Scholar 

  • Hirai MY, Klein M, Fujikawa Y, Yano M, Goodenowe DB, Yamazaki Y, Kanaya S, Nakamura Y, Kitayama M, Suzuki H, Sakurai N, Shibata D, Tokuhisa J, Reichelt M, Gershenzon J, Papenbrock J, Saito K. Elucidation of gene-to-gene and metabolite-to-gene networks in Arabidopsis by integration of metabolomics and transcriptomics. J Biol Chem. 2005;280:25590–5.

    Article  CAS  PubMed  Google Scholar 

  • Huhman DV, Sumner LW. Metabolic profiling of saponin glycosides in Medicago sativa and Medicago truncatula using HPLC coupled to an electrospray ion-trap mass spectrometer. Phytochemistry. 2002;59:347–60.

    Article  CAS  PubMed  Google Scholar 

  • Lepiniec L, Debeaujon I, Routaboul JM, Baudry A, Pourcel L, Nesi N, Caboche M. Genetics and biochemistry of seed flavonoids. Annu Rev Plant Biol. 2006;57:405–30.

    Article  CAS  PubMed  Google Scholar 

  • Nagel J, Culley LK, Lu YP, Liu EW, Matthews PD, Stevens JF, Page JE. EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol. Plant Cell. 2008;20:186–200.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yonekura-Sakakibara K, Tohge T, Niida R, Saito K. Identification of a flavonol 7-O-rhamnosyltransferase gene determining flavonoid pattern in Arabidopsis by transcriptome coexpression analysis and reverse genetics. J Biol Chem. 2007;282:14932–41.

    Article  CAS  PubMed  Google Scholar 

  • Yonekura-Sakakibara K, Tohge T, Matsuda F, Nakabayashi R, Takayama H, Niida R, Watanabe-Takahashi A, Inoue E, Saito K. Comprehensive flavonol profiling and transcriptome coexpression analysis leading to decoding gene-metabolite correlations in Arabidopsis. Plant Cell. 2008;20:2160–76.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ziegler J, Voigtlander S, Schmidt J, Kramell R, Miersch O, Ammer C, Gesell A, Kutchan TM. Comparative transcript and alkaloid profiling in Papaver species identifies a short chain dehydrogenase/reductase involved in morphine biosynthesis. Plant J. 2006;48:177–92.

    Article  CAS  PubMed  Google Scholar 

  • Zywicki B, Catchpole G, Draper J, Fiehn O. Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic field-grown potatoes. Anal Biochem. 2005;336:178–86.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Guo-dong Wang .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Chemical Industry Press, Bei**g and Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Wang, Gd. (2015). Applications of LC-MS in Plant Metabolomics. In: Qi, X., Chen, X., Wang, Y. (eds) Plant Metabolomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9291-2_9

Download citation

Publish with us

Policies and ethics

Navigation