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Time-Resolved Macromolecular Crystallography in Practice at BioCARS, Advanced Photon Source: From Data Collection to Structures of Intermediates

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The Future of Dynamic Structural Science

Abstract

Time-resolved macromolecular crystallography is one of several “kinetic crystallography” methods (Bourgeois D, Royant A, Curr Opin Struct Biol 15:538–547, 2005; Bourgeois D, Weik M, Crystallogr Rev 15:87–118, 2009). In kinetic crystallography experiments, genuine biological function is triggered in the crystal with a goal of capturing molecules in action and determining structures of intermediate states. In time-resolved experiments in particular, short and intense X-ray pulses are used to probe intermediates in real time and at room temperature, in reactions that are initiated synchronously and rapidly in the crystal. We provide here an overview of time-resolved crystallography as implemented today at the BioCARS beamline 14-ID at the Advanced Photon Source (Graber T et al., J Synchrotron Radiat 18:658–670, 2011), with an overview of future directions and challenges.

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Authors and Affiliations

  1. Center for Advanced Radiation Sources, The University of Chicago, Chicago, IL, 60637, USA

    Vukica Šrajer

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  1. Vukica Šrajer
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Correspondence to Vukica Šrajer .

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Editors and Affiliations

  1. Department of Chemistry, Durham University, Durham, United Kingdom

    Judith A.K. Howard

  2. School of Chemistry, University of Bristol, Bristol, United Kingdom

    Hazel A. Sparkes

  3. Department of Chemistry, University of Bath, United Kingdom

    Paul R. Raithby

  4. N.S. Kurnakov Institute of General and Inorganic Chemistry Russian Academy of Sciences, Moscow, Russia

    Andrei V. Churakov

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Šrajer, V. (2014). Time-Resolved Macromolecular Crystallography in Practice at BioCARS, Advanced Photon Source: From Data Collection to Structures of Intermediates. In: Howard, J., Sparkes, H., Raithby, P., Churakov, A. (eds) The Future of Dynamic Structural Science. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8550-1_17

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