Abstract
The granulosa cell has an important physiological role in supporting the formation of ovarian follicles and the maturation of oocytes and early embryos. We have developed an improved serum-free medium to optimize the cell growth of bovine granulosa cells (BGC). BGC seeded on collagen-coated culture plates proliferated progressively in a serum-free medium supplemented with insulin, heparin binding growth factor-2 (HBGF-2), lipoprotein (LP), and BSA. The cell doubling time at logarithmic phase and final cell density were equal to those in serum-containing medium. Extensive cell proliferation of BGC was primarlily important to induce the early bovine embryo development in vitro. When embryos were co-cultured with BGC in a defined medium, insulin and HBGF-2 stimulated embryonic development to the blastocyst stage as effective as serum did. Denuded embryos (without granulosa cells) could not enter into the blastocyst stage even in the presence of insulin and HBGF-2. These results suggest that insulin and HBGF-2 stimulate early bovine embryonic development by activating BCC function.
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© 1991 Springer Science+Business Media Dordrecht
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Hoshi, H., Takagi, Y., Kobayashi, K., Onodera, M., Oikawa, T. (1991). Growth and Function of Bovine Granulosa Cells Cultured in a Serum-Free Medium. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_24
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DOI: https://doi.org/10.1007/978-94-011-3550-4_24
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