Abstract
DNA was extracted from a variety of chemically-transformed mouse fibroblast lines, and used as a donor for the transfection of NIH3T3 cells. Some of these DNAs were able to induce the appearance of transformed foci on the NIH3T3 monolayer following transfection. These foci grow in soft agar and are tumorigenic in newborn mice. The ability to tag and modify the DNA prior to its transfection, allowed us to initiate the molecular cloning of the transforming gene from one of the chemically transformed lines. The experimental cloning strategy currently underway can be used as a general strategy for other single-copy selectable cellular genes.
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References
McCann, J., Chai, E., Yamasaki, E. and Ames, B.N. (1975) Proc. Natl. Acad. Sci. USA 72, 5135.
McCann, J. and Ames, B.N. (1976) Proc. Natl. Acad. Sci. USA 73, 950.
Bridges, B.A. (1976) Nature (London) 261, 195.
Bouck, N. and diMayorka, G. (1976) Nature (London) 264, 722.
Graham, F.L. and van der Eb, A.J. (1973) Virology 52, 456.
Wigler, M., Pellicer, A., Silverstein, S. and Axel, R. (1978) Cell 14, 725.
Wigler, M., Sweet, R., Sim, G.K., Wold, B., Pellicer, A., Lacy, E., Maniatis, T., Silverstein, S. and Axel, R. (1979) Cell 16, 777.
Hill, M., Hillova, J., Dantcher, D., Mariage, R. and Goubin, C. (1974) Cold Spring Harbor Symp. Quant. Biol. 39,. 1015.
Cooper, G.M. and Temin, H.M. (1974) J. Virol 14, 1132.
Smotkin, D., Gianni, A.M., Rozenblatt, S. and Weinberg, R.A. (1975) Proc. Natl. Acad. Sci. USA 72, 4910.
Anderason, P., Goldfarb, M.P. and Weinberg, R.A. (1979) Cell 16, 63.
Goldfarb, M.P. and Weinberg, R.A. (1979) J. Virol 32, 30.
Goldfarb, M.P. and Weinberg, R.A., submitted for publication.
Shih, C., Shilo, B., Goldfarb, M.P., Dannenberg, A. and Weinberg, R.A. (1979) Proc. Natl. Acad. Sci. USA 76, 5714.
Cooper, G.M., Okenquist, S. and Silverman, L. (1980) Nature(London) 284, 418.
Reznikoff, C.A., Bertram, J.S., Brankow, D.W. and Heidelberger, C. (1973) Cancer Research 33, 3239.
Hicks, J.B., Hinnen, A. and Fink, G.R. (1979) Cold Spring Harbor Symp. Quant. Biol. 43, 1305.
Southern, E.M. (1975) J. Mol. Biol. 98, 503.
Shilo, B., Shih, C., Merrick, M., Dannenberg, A., Goldfarb, M.P. and Weinberg, R.A. 12th Miami Winter Symposium, in press.
Maniatis, T., Hardison, R.C., Lacy, E., Laver, J., O’Connel, C., Quon, D., Sim, G.K and Eistratiadis, A. (1978) Cell 15, 687.
Benton, W.D. and Davis, R.W. (1977) Science 196, 180.
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Shilo, BZ., Shih, C.H., Merrick, M., Padhy, L.C., Weinberg, R.A. (1980). Progress in Cloning the Transforming Gene from Chemically-Transformed Mouse Cells. In: Pullman, B., Ts’o, P.O.P., Gelboin, H. (eds) Carcinogenesis: Fundamental Mechanisms and Environmental Effects. The Jerusalem Symposia on Quantum Chemistry and Biochemistry, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-9104-0_44
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DOI: https://doi.org/10.1007/978-94-009-9104-0_44
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