Summary
The motility of ram cauda epididymal spermatozoa exposed to 1 mM- RS α-chlorohydrin and their ATP concentration declined dramatically after 15 min incubation with 5 mM-D glucose or 5 mM-D glucose + 10 mM- L lactate + 1 mM-pyruvate. By contrast the effects of α-chlorohydrin were relatively slight in the absence of glucose. The concerted action of α-chlorohydrin and glucose may dissipate energy in a ‘futile cycling’ reaction and this could explain the effective contraceptive action of the drug.
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References
Brown-Woodman PDC, Mohri H, Mohri T,Suter D, White IG (1978) Mode of action of a-chlorohydrin as an antifertility agent. Biochem. J. 170, 23–37
Ford WCL (1982) The Mode of Action of 6-chloro-6-deoxysugars as antifertility agents in the male. In Progress towards a male contraceptive. Ed. By SL Jeffcoate amp; M Sandlers. In press. Chichester, John Wiley
Ford WCL, Harrison A (1981) Effects of 6-chloro-6-deoxysugars on adenine nucleotide concentrations in and motility of rat spermatozoa. J. Reprod. Fert. 63, 75–79
Ford WCL, Harrison A, Waites GMH (1981) Effects of 6-chloro-6-deoxysugars on glucose oxidation in rat spermatozoa. J. Reprod. Fert. 75–79
Fraser LR, Quinn PJ (1981) A glycolytic products is obligatory for initiation of the sperm acrosome reaction and whiplash motility required for fertility in the mouse. J. Reprod. Fert. 61, 25–35
Katz J, Rognstad R (1976) Furtile cycles in the metabolism of glucose. Curr. Topics Cell Reg. 10, 238–289
Sokoloski JE, Blasco L, Storey BT, Wolf DP (1977) Turbidimetric analysis of human sperm motility. Fert. Steril. 28, 1337–1341
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© 1983 Martinus Nijhoff Publishers, The Hague
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Ford, W.C.L., Harrison, A. (1983). The Effect of Different Substrate Combinations on the Survival of Ram Cauda Epididymal Spermatozoa exposed to 1 mM-RS α-Chlorohydrin. In: André, J. (eds) The Sperm Cell. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7675-7_13
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DOI: https://doi.org/10.1007/978-94-009-7675-7_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-009-7677-1
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