Abstract
We report on a survey of apolipoprotein A-I and B measurements among 11 laboratories using a lyophilized serum to be evaluated for its possible use as “reference serum” in a large prospective study. Using a single method, radial immunodiffusion (RID), and a common antibody the partecipating laboratories evaluated the apo A-I and apo B concentrations in the proposed reference serum. Analyses were performed in quadruplicate in four consecutive days to estimate the day by day variation in a given laboratory. This value resulted to be 2% for apo A-I and 1.9% for apo B.
The grand mean values for apo B (92 mg/dl) and A-I (131 mg/dl) were very similar to those obtained with the same methods in the central laboratory. The serum was stable up to 1 year, thus it may be used as quality control serum in the determination of apo B and apo A-I concentrations for this study.
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© 1990 Kluwer Academic Publishers
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Roma, P. et al. (1990). A survey on apolipoprotein A-I and B: the CNR study. In: Descovich, G., Gaddi, A., Magri, G., Lenzi, S. (eds) Atherosclerosis and Cardiovascular Disease. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0731-7_69
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DOI: https://doi.org/10.1007/978-94-009-0731-7_69
Publisher Name: Springer, Dordrecht
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