Abstract
Helper-dependent adenoviral vector has demonstrated a high therapeutic potential; however, the poor efficiency of the actual production process hampers further large-scale clinical evaluation. In this work, we evaluated the adenofection protocol, an efficient polyethylenimine transfection strategy, for HDV production. Optimized conditions of PEI-adenofection were used to generate HDV at 3-L bioreactor scale. This process strategy realized in suspension reduced process duration and therefore ensured production of high-quality vector stock.
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References
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Acknowledgments
E.D. acknowledges the MCETC/FRSQ/CIHR Strategic training program in Cancer Experimental Therapeutics and the Swiss National Science Foundation (PBSK2–115853). The authors thank Dr. P. Lowenstein for the HV gift; S. Perret, G. St-Laurent, A. Bernier, R. Tom, N. Arcand and L. Bourget for technical support; and M. Aucoin for critical comments.
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Dormond, E., Meneses-Acosta, A., Jacob, D., Durocher, Y., Perrier, M., Kamen, A. (2010). A Scalable Process for Helper-Dependent Adenoviral Vector Production Using PEI-Derived Transfection Strategy in Suspension Culture. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_77
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DOI: https://doi.org/10.1007/978-90-481-3419-9_77
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