Abstract
The purpose of this study was to examine the effect of osteoblastic differentiation of mesenchymal stem cell (MSC) on new bone formation in β-TCP/MSC complex in vivo. Bone marrows were collected from beagle and cultured. Cultured cells were implanted into β-TCP. β-TCP/MSC complexes and control materials were surgically implanted into dorsal pouches of beagle. Eight weeks after the surgery, the implanted materials were harvested and evaluated. In all materials, new bone formation was observed. A significant increase in new bone formation was seen in non-differentiation complex and 1-day differentiation complex as compared with the control. However, new bone formation in 7-day differentiation complex was less than the other two complexes. The results suggest that the implantation of MSC intoβ-TCP enhances new bone formation, but the effect of osteoblastic differentiation of MSC is rather inhibitory.
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References
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Kubota, M., Tokugawa, Y., Nishimura, M., Igarashi, K. (2007). New bone formation in β-TCP/MSC complex: effect of osteoblastic differentiation of MSC. In: Watanabe, M., Okuno, O., Sasaki, K., Takahashi, N., Suzuki, O., Takada, H. (eds) Interface Oral Health Science 2007. Springer, Tokyo. https://doi.org/10.1007/978-4-431-76690-2_82
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DOI: https://doi.org/10.1007/978-4-431-76690-2_82
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-76689-6
Online ISBN: 978-4-431-76690-2
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