Abstract
Previous studies have described alterations in gene expression following spinal cord injury, but this response to mechanical stimuli is difficult to investigate in vivo. Therefore, we have investigated the effect of cyclic tensile strain on cultured spinal cord cells from E15 Sprague–Dawley rats. Microarray analysis of gene expression and categorization of identified genes were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) systems.
The application of cyclic tensile strain reduced the viability of cultured spinal cord cells significantly in a dose- and time-dependent manner. GO analysis identified candidate genes related to “apoptosis” (44) and to “response to stimulus” (17). KEGG analysis identified changes in the expression levels of 12 genes of the mitogen-activated protein kinase (MAPK) signaling pathway, which were confirmed to be upregulated and validated by RT-PCR analysis. Spinal cord cells undergo cell death in response to cyclic tensile strain, which were dose- and time-dependent, with upregulation of various genes, in particular of the MAPK pathway.
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Acknowledgment
This work was supported in part by Grants-in-Aid for General Scientific Research of the Ministry of Education, Science and Culture of Japan (grants numbers 16390435, 18390411, 19791023, 21591895, and 21791389). This study was also supported by grants from Research Program of Spinal Cord Intractable Pain (2009), The Public Health Bureau of the Japanese Ministry of Health and Welfare.
Conflict of Interest All authors declare that they have no conflict of interest.
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Uchida, K., Nakajima, H., Hirai, T., Roberts, S., Johnson, W.E.B., Baba, H. (2014). Microarray Analysis of Expression of Cell Death-Associated Genes in Spinal Cord Cells with Cyclic Tensile Strain. In: Uchida, K., Nakamura, M., Ozawa, H., Katoh, S., Toyama, Y. (eds) Neuroprotection and Regeneration of the Spinal Cord. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54502-6_11
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DOI: https://doi.org/10.1007/978-4-431-54502-6_11
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