Abstract
Lipid-free polysaccharides generally are not attached to the surface of erythrocytes. In contrast O-stearoyl derivatives of polysaccharides show a high affinity for red blood cells. By reaction of polysaccharides with stearoyl chloride in dimethyl formamide as a solvent in the presence of pyridine, derivatives are obtained which exhibit marked erythrocyte-sensitizing activity. The chemical conditions for optimal sensitization of erythrocytes were investigated for a number of polysaccharides, e.g. starch, dextran, and several lipid-free bacterial cell wall polysaccharides. A content of about 5% by weight of O-stearoyl groups has been found most suitable for producing erythrocyte-sensitizing properties without modification of the serological specificity of the polysaccharide.
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Abbreviations
- DMF:
-
Dimethyl formamide
- PBS:
-
phosphate-buffered saline
References
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Hämmerling, U., Westphal, O. (1967). Synthesis and Use of O-Stearoyl Polysaccharides in Passive Hemagglutination and Hemolysis. In: Liébecq, C. (eds) European Journal of Biochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-25813-2_9
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DOI: https://doi.org/10.1007/978-3-662-25813-2_9
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