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Cryopreservation of Humulus lupulus L. (Hop)

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Cryopreservation of Plant Germplasm II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 50))

Abstract

The cultivated hop (Humulus lupulus, Cannabinaceae) is a hardy herbaceous climbing plant that is indigenous in the northern hemisphere above 32° latitude. Germany, the USA, China and the Czech Republic are the main producer countries. The Spanish production represents around 5% of the total production of the European Union, having the third place in importance among the producing member countries behind Germany and the United Kingdom.

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References

  • Adams AN (1975) Elimination of viruses from the hop (Humulus lupulus) by heat therapy and meristem culture. J Hortic Sci 50: 151–160

    Google Scholar 

  • Arora R, Wisniewki ME (1994) Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica L. Batsch ). Plant Physiol 105: 95–101

    Article  PubMed  CAS  Google Scholar 

  • Bachiri Y, Gazeau C, Hansz J, Morisset C, Dereuddre J (1995) Successful cryopreservation of suspension cells by encapsulation-dehydration. Plant Cell Tissue Organ Cult 43: 241–248

    Google Scholar 

  • Benson EE, Reed BM, Brennan RM, Clacher KA, Ross DA (1996) Use of thermal analysis in the evaluation of cryopreservation protocols for Ribes nigrum L. germplasm. Cryo Lett 17: 347–362

    Google Scholar 

  • Brady JL, Scott NS, Thomas MR (1996) DNA ty** of hops (Humulus lupulus) through application of RAPD and microsatellite marker sequences converted to sequence tagged sites ( STS ). Euphytica 91: 277–284

    Google Scholar 

  • Daie J, Campbell WF (1981) Response of tomato plants to stressful temperatures. Plant Physiol 67: 26–29

    Article  PubMed  CAS  Google Scholar 

  • Dereuddre J (1992) Cryopreservation of in vitro cultures of plant cells and organs by vitrification and dehydration. In: Dattée Y, Dumas C, Gallais A (eds) Reproductive biology and plant breeding. Springer, Berlin Heidelberg New York, pp 291–300

    Chapter  Google Scholar 

  • Dereuddre J, Scottez C, Arnaud Y, Duron M (1990) Résistance d’apex axillaires de poirer (Pyrus communis L.) à une déshydratation puis à une congélation dans l’azote liquide: effet d’un traitement au froid des vitroplants. CR Acad Sci Paris 310 (Ser III): 317–323

    Google Scholar 

  • Dereuddre J, Hassen N, Blandin S, Kaminski M (1991) Resistance of alginate-coated somatic embryos of carrot (Daucus carota L.) to desiccation and freezing in liquid nitrogen. 2. Thermal analysis. Cryo Lett 12: 135–148

    Google Scholar 

  • Dumet D, Engelmann F, Chanbrillange N, Dereuddre J (1993) Importance of sucrose for the acquisition of tolerance to desiccation and cryopreservation of oil palm somatic embryos. Cryo Lett 14: 243–250

    Google Scholar 

  • Fric V, Krofta K, Svoboda P, Kopecky J (1997) The evaluation of the virus-free hop quality after five years growing in the Czech Republic. Rostlinna Vyroba 43: 307–314

    Google Scholar 

  • Fukai S, Togashi M, Goi M (1994) Cryopreservation of in vitro-grown Dianthus by encapsulation-dehydration. Tech Bull Fac Agric Kagawa Univ 46: 101–107

    Google Scholar 

  • Gonzalez-Arnao MT, Moreira T, Urra C (1996) Importance of pregrowth with sucrose and vitrification for the cryopreservation of sugarcane apices using encapsulation-dehydration. Cryo Lett 117: 141–148

    Google Scholar 

  • Haunold A (1981) Hop production, breeding and variety development in various countries. J Am Soc Brew Chem 39: 27–34

    Google Scholar 

  • Heale JB, Legg T, Connell S (1989) Humulus lupulus L. (hop): in vitro culture; attempted production of bittering components and novel disease resistance. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 7. Medicinal and aromatic plants II. Springer, Berlin Heidelberg New York, pp 264–285

    Google Scholar 

  • Kohmura H, Ikeda Y, Sakai A (1994) Cryopreservation of apical meristems of Japanese shallot (Allium wakegi A.) by vitrification and subsequent high plant regeneration. Cryo Lett 15: 289–298

    Google Scholar 

  • Mari S, Engelmann F, Chabrillange N, Huet C, Micchaux-Ferrièe N (1995) Histo-citological study of apices of coffee (Coffea racemosa and C. sessiliflora) in vitro plantlets during their cryopreservation using the encapsulation-dehydration technique. Cryo Lett 16: 289–298

    Google Scholar 

  • Martínez D (1999) Crioconservación de apices de tallos de lúpulo: analisis térmico. Tesis Doctoral, Universidad de Oviedo, Oviedo, Spain

    Google Scholar 

  • Martínez D, Revilla MA (1998) Cold acclimation and thermal transitions in the cryopreservation of hop shoot tips. Cryo Lett 19: 333–342

    Google Scholar 

  • Martínez D, Revilla MA, Espina A, Jaimez E, García JR (1998) Survival cryopreservation of hop shoot tips monitored by differential scanning calorimetry. Thermochim Acta 317: 91–94

    Article  Google Scholar 

  • Martínez D, Tamés RS, Revilla MA (1999) Cryopreservation of in vitro grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration. Plant Cell Rep 19: 59–63

    Article  Google Scholar 

  • Matousek J, Junker V, Vrba L, Schubert J, Patzak J, Steger G (1999) Molecular characterization and genome organization of 7SL RNA genes from hop (Humulus lupulus L.). Gene 239: 173–183

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Neve RA (1991) Hops. Chapman and Hall, London, pp 1–10

    Book  Google Scholar 

  • Niino T, Sakai A (1992) Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear, and mulberry. Plant Sci 87: 199–206

    Article  CAS  Google Scholar 

  • Niino T, Sakai A, Nojiri K (1992) Cryopreservation of in vitro-grown shoot tips of apple and pear by vitrification. Plant Cell Tissue Organ Cult 28: 261–266

    Article  Google Scholar 

  • Panis B (1995) Cryopreservation of banana (Musa spp.) germplasm. Dissertatons de Agricultura, Katholieke Universitiet, Leuven, Belgium

    Google Scholar 

  • Panis B, Totté N, Nimmen KV, Withers LA, Swennen R (1996) Cryopreservation of banana (Musa spp.) meristem cultures after preculture on sucrose. Plant Sci 121: 95–106

    Article  CAS  Google Scholar 

  • Paulet F, Engelmann F, Glaszmann J (1993) Cryopreservation of apices of in vitro plantlets of sugarcane (Saccharum sp. hybrids) using encapsulation/dehydration. Plant Cell Rep 12: 525–529

    Article  Google Scholar 

  • Pillay M, Kenny ST (1996a) Structure and inheritance of ribosomal DNA variants in cultivated and wild hop, Humulus lupulus L. Theor Appl Genet 93: 333–340

    Article  CAS  Google Scholar 

  • Pillay M, Kenny ST (1996b) Random amplified polymorphic DNA (RAPD) markers in hop, Humulus lupulus: level of genetic variability and segregation in F1 progeny. Theor Appl Genet 92: 334–339

    Article  CAS  Google Scholar 

  • Plessis P, Leddet C, Collas A, Dereuddre J (1993) Cryopreservation of Vitis vinifera L. cv. Chardonnay shoot tips by encapsulation-dehydration: effects of pretreatment, cooling and postculture conditions. Cryo Lett 14: 309–320

    Google Scholar 

  • Probasco G, Winslow S (1986) The use of shoot-tip culture to eliminate viruses from hop varieties grown in the United States. MBAA Tech Q 23: 26–31

    Google Scholar 

  • Redenbaugh K, Paasch BD, Nichol JW, Kessler ME, Viss PR, Walker KA (1986) Somatic seeds: encapsulation of asexual plant embryos. Biotech 4: 797–801

    Article  Google Scholar 

  • Sakai A, Kobayashi,S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9: 30–33

    Google Scholar 

  • Salmon ES (1934) Two new hops: Brewer’s Favourite and Brewer’s Gold. Agric Coll Wye 34: 93–105

    Google Scholar 

  • Samyn G, Welvaert W (1983) Producing a “nuclear stock” of virus-free hop plants. Med Fac Land Bouww Rijsunic Gent 48: 877–881

    Google Scholar 

  • Scottez C, Chevreau E, Godard N, Arnaud Y, Duron M, Dereuddre J (1992) Cryopreservation of cold acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration. Cryobiology 29: 691–700

    Article  Google Scholar 

  • Sharom M, Willemot C, Thompson J (1994) Chilling injury induces changes in membranes of tomato fruit. Plant Physiol 105: 305–308

    PubMed  CAS  Google Scholar 

  • Vandenbussche B, Demeulemeester MAC, De Proft MP (1993) Cryopreservation of alginate-coated in vitro grown shoot-tips of chicory (Cichorium intybus L.) using rapid freezing. Cryo Lett 14: 259–266

    Google Scholar 

  • Vine SJ, Jones OP (1969) The culture of shoot tips of hop (Humulus lupulus L.) to eliminate viruses. J Hortic Sci 44: 281–284

    Google Scholar 

  • Wetmore RH, Sorokin S (1955) On the differentiation of xylem. J Arnold Arboretum 36: 305–317

    Google Scholar 

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Author information

Authors and Affiliations

  1. Departamento Biología de Organismos y Sistemas, Facultad de Biología, C/C. Rodrigo Uría s/n, Universidad de Oviedo, 33071, Oviedo, Spain

    M. A. Revilla & D. Martínez

Authors
  1. M. A. Revilla
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  2. D. Martínez
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Editor information

Editors and Affiliations

  1. USDA-ARS National Center for Genetic Resources Preservation, 1111 S. Mason St., 80521, Fort Collins, CO, USA

    Leigh E. Towill

  2. New Delhi, India

    Y. P. S. Bajaj

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© 2002 Springer-Verlag Berlin Heidelberg

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Revilla, M.A., Martínez, D. (2002). Cryopreservation of Humulus lupulus L. (Hop). In: Towill, L.E., Bajaj, Y.P.S. (eds) Cryopreservation of Plant Germplasm II. Biotechnology in Agriculture and Forestry, vol 50. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04674-6_10

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  • DOI: https://doi.org/10.1007/978-3-662-04674-6_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-07502-5

  • Online ISBN: 978-3-662-04674-6

  • eBook Packages: Springer Book Archive

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