Experiments on Na Transport of Frog Skin Epithelium

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Laboratory Techniques in Membrane Biophysics
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Abstract

The experiments were chosen for their educational value and have two objectives. First, active transport will be demonstrated in terms of the short circuit current (SCC). The ionic species transported will be identified by comparing the SCC with the net flux of sodium as measured by tracer techniques. Second, hormonal influence of active transport (SCC) will be observed. The hormonal effect will be analyzed in more detail in terms of a compartmental model of frog-skin.

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References

  1. Ussing, H. H.: Acta physiol. scand. 19, 43 (1949).

    Article  CAS  Google Scholar 

  2. Schlögl, R.: Habilitationsschrift. Göttingen 1957.

    Google Scholar 

  3. Ussing, H. H., and K. Zerahn: Acta physiol. scand. 23, 110 (1951).

    Article  PubMed  CAS  Google Scholar 

  4. Curran, P. F., F. C. Herrera, and W. J. Flamigan: J. gen. Physiol. 46, 1011 (1963).

    Article  PubMed  CAS  Google Scholar 

  5. Cereijido, M., F. C. Herrera, W. J. Flamigan, and P. F. Curran: J. gen. Physiol. 47, 879 (1964).

    Article  PubMed  CAS  Google Scholar 

  6. Schoffeniels, E.: Biochem. biophys. Acta (Amst.) 26, 585 (1957).

    Article  CAS  Google Scholar 

  7. Hoshiko, T., and H. H. Ussing: Acta physiol. scand. 49, 74 (1960).

    Article  PubMed  CAS  Google Scholar 

  8. Rehm, W. S., and W. H. Dennis: Metabolic aspects of transport across cell membranes. U. of Wisconsin Press 1957.

    Google Scholar 

  9. Schultz, S. G.: J. gen. Physiol. 47, 567, 1043 (1964);

    Article  PubMed  CAS  Google Scholar 

  10. Schultz, S. G.: J. gen. Physiol. 48, 375 (1965).

    Article  Google Scholar 

  11. Clarkson, T. W., and S. R. Toole: Amer. J. Physiol. 206, 658 (1964).

    CAS  Google Scholar 

  12. Ussing, H. H., and B. Anderson: Proc. 3rd intern. Congress Biochem. Brussels, p. 434 (1955).

    Google Scholar 

  13. Cooperstein, I. L., and C. A. Hogben: J. gen. Physiol. 42, 461 (1959).

    Article  PubMed  CAS  Google Scholar 

  14. Leaf, A., J. Anderson, and L. B. Page: J. gen. Physiol. 41, 657 (1958).

    Article  PubMed  CAS  Google Scholar 

  15. Eigler, F. W., U. Held, W. Karger, and K. J. Ullrich: Pflügers Arch. ges. Physiol. 270, 79 (1959).

    Google Scholar 

  16. Donn, A., and D. M. Maurice: Arch. Ophthal. 62, 741, 748 (1959).

    Article  PubMed  CAS  Google Scholar 

  17. Cole, D. F.: Brit. J. Ophthal. 46, 577 (1962).

    Article  PubMed  CAS  Google Scholar 

  18. Cole, K. S.: Arch. Sci. physiol. 3, 253 (1949).

    CAS  Google Scholar 

  19. Marmont, G.: J. cell. comp. Physiol. 34, 351 (1949).

    Article  CAS  Google Scholar 

  20. Hodgkin, A. L., A. F. Huxley, and B. Katz: J. Physiol. (Lond.) 116, 424 (1952).

    CAS  Google Scholar 

  21. Hays, R. M., and A. Leaf: J. gen. Physiol. 45, 905, 921, 933 (1962).

    PubMed  Google Scholar 

  22. Essig, A., and A. Leaf: J. gen. Physiol. 46, 505 (1963).

    Article  PubMed  CAS  Google Scholar 

  23. Anderson, B., and K. Zerahn: Acta physiol. scand. 59, 319 (1963).

    Article  Google Scholar 

  24. Ussing, H. H., T. U. L. Bieber, and N. S. Bricker: J. gen. Physiol. 48, 425 (1964).

    Article  Google Scholar 

  25. Lindley, B. D., and T. Hoshiko: J. gen. Physiol. 47, 773 (1964).

    Article  PubMed  CAS  Google Scholar 

  26. Finkelstein, A.: J. gen. Physiol. 47, 545 (1964).

    Article  PubMed  CAS  Google Scholar 

  27. Ussing, H. H., and E. E. Windhager: Acta physiol. scand. 61, 484 (1964).

    PubMed  CAS  Google Scholar 

  28. Curran, P. F., and M. Cereijido: J. gen. Physiol. 48, 543, 1011 (1965).

    Article  PubMed  CAS  Google Scholar 

  29. Ussing, H. H.: Acta physiol. scand. 63, 141 (1965).

    Article  PubMed  CAS  Google Scholar 

  30. Bullivant, S., and W. R. Loewenstein: J. Cell Biol. 37, 621 (1968).

    Article  PubMed  CAS  Google Scholar 

  31. Gatzy, J. T.: Fed. Proc. 25, 507 (1966).

    Google Scholar 

  32. Tormey, J. McD., and J. M. Diamond: J. gen. Physiol. 50, 2031 (1967).

    Article  PubMed  CAS  Google Scholar 

  33. Ussing, H. H.: J. Cell Biol. 36, 625 (1968).

    Article  PubMed  Google Scholar 

  34. Lindemann, B.: Biochim. biophys. Acta (Amst.) 163, 424 (1968).

    Article  CAS  Google Scholar 

  35. Bueno, E. J., and L. C’orchs: J. gen. Physiol. 51, 785 (1968).

    Article  PubMed  CAS  Google Scholar 

  36. Hays, R. M.: J. gen. Physiol. 51, 385 (1968).

    Article  PubMed  CAS  Google Scholar 

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Clarkson, T.W., Lindemann, B. (1969). Experiments on Na Transport of Frog Skin Epithelium. In: Passow, H., Stämpfli, R. (eds) Laboratory Techniques in Membrane Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87259-4_9

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  • DOI: https://doi.org/10.1007/978-3-642-87259-4_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-04592-2

  • Online ISBN: 978-3-642-87259-4

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