Abstract
We have found a novel stress (heat shock) protein whose molecular size is 47kDa. As hsp47 binds to collagen, it can be easily purified using gelatin-Sepharose. Indirect immunofluorescence and immunoelectron microscopic studies using polyclonal and monoclonal antibodies against HSP47 indicated the localization of HSP47 in the endoplasmic reticulum (ER) of the fibroblast. Immunoprecipitation studies using anti-HSP47 antibody revealed that two chains (α and ß) of type I procollagen were co-precipitated with HSP47.
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© 1991 Springer-Verlag Berlin Heidelberg
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Nagata, K. et al. (1991). Interaction of HSP47 with Newly Synthesized Procollagen, and Regulation of HSP Expression. In: Maresca, B., Lindquist, S. (eds) Heat Shock. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76679-4_11
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DOI: https://doi.org/10.1007/978-3-642-76679-4_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-76681-7
Online ISBN: 978-3-642-76679-4
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