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Primary and Secondary Cultures of Rabbit Articular Chondrocytes in a Serum-Free Medium

  • Conference paper
Hormonally Defined Media

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

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Abstract

Cell growth and function is partly under the control of hormones and growth factors. Their effect on cartilaginous tissue was studied by develo** a serum free medium in which chondrocytes are able to grow inculture. The defined medium contains various spreading and nutrient factors: BSA, bovine fibronectin, transferrin, thrombin, selenium as well as hormones and growth factors: insulin, hydrocortisone, MSA, BGF or FGF. Growth stimulation in this medium was largely due to the growth factors used. This medium does not affect the specific function of type II collagen synthesis.

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Literature references

  1. Green W.T. Behaviour of articular chondrocytes in culture; Clin. Orthop., 1971, 75, 248.

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Author information

Authors and Affiliations

  1. Laboratoire de Pharmacologie cellulaire de l’E. P. H. E., Institut Biomédical des Cordemiers, F-75006, Paris, France

    M. Adolphe, X. Ronot, B. Froger, M. T. Corvol & N. Forest

Authors
  1. M. Adolphe
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  2. X. Ronot
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  3. B. Froger
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  4. M. T. Corvol
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  5. N. Forest
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Editor information

Editors and Affiliations

  1. Institut für Neurobiologie, Universität Heidelberg, Im Neuenheimer Feld 504, 6900, Heidelberg 1, Germany

    G. Fischer

  2. Institut für Pharmakologie, Universität Mainz, Obere Zahlbacher Str. 67, 6500, Mainz, Germany

    R. J. Wieser

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© 1983 Springer-Verlag Berlin Heidelberg

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Adolphe, M., Ronot, X., Froger, B., Corvol, M.T., Forest, N. (1983). Primary and Secondary Cultures of Rabbit Articular Chondrocytes in a Serum-Free Medium. In: Fischer, G., Wieser, R.J. (eds) Hormonally Defined Media. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69290-1_53

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  • DOI: https://doi.org/10.1007/978-3-642-69290-1_53

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69292-5

  • Online ISBN: 978-3-642-69290-1

  • eBook Packages: Springer Book Archive

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