Abstract
A series of six monoclonal antibodies reactive with hematopoietic differentiation antigens expressed at different stages of myelocytic and erythrocytic differentiation have been developed. One of these antibodies (IG10) is reactive mainly with mature and immature granulocytes, including CFU-GM. Four of these antibodies (T5A7, T3A3, L4F3, L182) are reactive with both granulocytes and monocytes and can be used to define distinct stages in granulocytic maturation. All are reactive with CFU-GM. The sixth antibody (5F1) is reactive with monocytic and nucleated erythrocytic elements, including CFU-E. Of particular interest is the observation that antibody 5F1 in the absence of complement inhibits CFU-E growth. None of these antibodies react with peripheral blood or bone marrow lymphocytes, thymocytes or erythrocytes. Potential use of such reagents in studies of normal and malignant hematopoietic differentiation will be discussed.
Reagents kindly supplied by: 1Dr. I. Trowbridge, 2Dr. S. Schlossman, 3Dr. L. Olsson; supported by the swedish cancer society and the king Gustav Vth jub. Foundation
For the generous provision of antibodies we gratefully acknowledge Dr. S. Schlossman for B1 and B2, Dr. E. Clark for BB-1 and LB-1 and Dr. J. Wiels for 38.13
The Norwegian Hydro Company is gratefully acknowledged its support of this study
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Andrews, R.G. et al. (1984). Leucocyte Malignancies. In: Bernard, A., Boumsell, L., Dausset, J., Milstein, C., Schlossman, S.F. (eds) Leucocyte Ty**. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68857-7_74
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