Summary
We have developed a new fabrication method for building large arrays of needles with nanometer-size apertures. Because the process is entirely based on standard photolithographic techniques and conventional silicon-based processing, large arrays can be obtained with a fast turnaround. The arrays can be easily integrated with on-chip microfluidics. Potential applications include the injection of substances into cells and also subcellular organelles such as vacuoles, mitochondria, and chloroplasts.
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References
M.R. Capecchi, “High efficiency transformation by direct microinjection of DNA into cultured mammalian cells,” Cell, vol. 22, pp. 479–488 (1980).
J.W. Gordon, G.A. Scangos, D.J. Plotkin, J.A. Barbosa, and F.H. Ruddle, “Genetic transformation of mouse embryos by microinjection of purified DNA,” Proc. Natl. Acad. Sci. USA, vol. 78, pp. 6376–6380 (1980).
L.N. Wei, “Transgenic animals as new approaches in pharmacological studies,” Ann. Rev. Pharmacol. Toxicol., vol. 37, pp. 119–141 (1997).
N.J.C. Lamb, R.C. Gauthier, and A. Fernandez, “Microinjection strategies for the study of mitogenic signaling in mammalian cells,” Frontiers in Bioscience, vol. 1, pp.D19–D29(1996).
M.M.H. Storms, C. Van Der Schoot, M. Prins, R. Kormelink, J.W.M. Van Lent, and R.W. Goldbach, “A comparison of two methods of microinjection for assessing altered plasmodesmal gating in tissues expressing viral movement proteins,” Plant J., vol. 13, pp. 131–140 (1998).
S.L. Jackson, “Microinjection of fungal cells: A powerful experimental technique,” Can. J. Bot., vol. 73(SUPPL. 1), pp. S435–S443 (1995).
K. Knoblauch, J.M. Hibberd, J.C. Gray, and A.J.E. van Bel, “A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes,” Nature Biotechnol., vol. 17, pp. 906–909 (1999).
N.W. Chang, and M.J. Wade, “The transfer of Wolbachia pipientis and reproductive incompatibility between infected and uninfected strains of the flour beetle, Tribolium confusum, by microinjection,” Can. J. Microbiol., vol. 40, pp. 978–981 (1994).
R. B. Marcus, T. S. Ravi, and T. Gmitter, “Formation of silicon tips with < 1 nm radius,” Appl. Phys. Lett., 56(3), pp. 236–238, Jan. 1990.
J. Nucci, H. Neves, Y. Shacham, E. Eisenbraun, B. Zheng, and A. Kaloyeros, “In-situ analysis of the microstructure of thermally treated thin copper films” Mat. Res. Soc. Symp. Proc, vol. 309, p. 377–382 (1993).
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Neves, H.P., Goldsmith, R.H., Degolyer, A.W., Bachand, G.D., Schmidt, J.J., Montemagno, C.D. (2001). Fabrication of a Nanoneedle Array. In: Obermeier, E. (eds) Transducers ’01 Eurosensors XV. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59497-7_250
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DOI: https://doi.org/10.1007/978-3-642-59497-7_250
Publisher Name: Springer, Berlin, Heidelberg
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