Abstract
To clarify the effects of Ultraviolet-B (UV-B) treatment on the primary energy transfer process in photosynthesis, PSII was exposed to UV-B radiation by two different methods. beside control samples, one are PSII particles isolated from plants that were irradiated each day for 8 h by increased UV-B (on top of natural light), second are PSII particles isolated from non-irradiated plants but exposed only to UV-B in vitro for 30 minutes directly before fluorescence spectrum was tested (the group of CK+UV-B30min). The steady-state fluorescence techniques were used, and Gaussian deconvolution analysis was employed to better identify the spectral changes resulting from UV-B radiation. The results showed that the group of CK+UV-B30min showed obviously fluorescence quenching for the degradation of some proteins in PSII, while the emission of the group of 7 day UV-B exposure was increased. The deconvolution analysis revealed that 30-min UV-B radiation changed the primary energy transfer process from Chlorophyll a (Chl a) to other pigments. However, if UV-B was added to plants for 7 day, photosystem performed overall regulation by re-arrangement of pigment-protein complexes to resist the stress, which not only altered the energy transfer process from Chl a but also Chlorophyll b (Chl b) and Carotenoid (Car).
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© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
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Liu, X., Yue, M., Ji, Q., He, J. (2013). Effects of Ultraviolet-B Radiation on Primary Photophysical Process in Photosystem II: a Fluorescence Spectrum Analysis. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_140
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DOI: https://doi.org/10.1007/978-3-642-32034-7_140
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