Abstract
In the present study a method was built based on liquid chromatography with diode array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface for the mainly active compounds in aqueous extracts from dried leaves of Apocynum venetum L. Overall, 10 constituents were separated and identified, which belong to two classes of compounds: caffeoylquinic acids and flavonoids. Among them, the qualitative and quantitative analysis of chlorogenic acid and cryptochlorogenic acid was the first report. 5 of them were further quantitatively analyzed. The contents of chlorogenic acid, cryptochlorogenic acid, hyperoside, isoquercitrin and astragalin were detected by this method as 2.1-3.9%, 0-0.71%, 1.1-2.2%, 0.47-0.87%, 0.19-0.35%, respectively. This method has a low detection limit with results range from 0.50 μg/mL to 1.5 μg/mL. Recovery was determined with standard addition method for sample, with results ranging from 94.2% to 106.1%. The data results provided a valuable reference to explore action mechanism of the true active constituents and improve the quality control for the herd Apocynum venetum L.
This work is supported by the National Science and Technology of key projects Grant# 2009ZX09310-005 to H.N. Liu, National Natural Science Foundation Grant#20962012 to X.Y. Yin, #30973959 to Q.S. Liu..
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Yin, Xy., Fu, Jj., Yang, Hs., Liu, Hn., Luo, Ym. (2012). Analysis of Flavonoids and Phenolic Acids of Aqueous Extracts in Leaves of Apocynum venetum L.. In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_110
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