Abstract
A pixeled plasmonic metasurface (MS) has been developed as a surface-enhanced infrared absorption (SEIRA) spectroscopy substrate to monitor the denaturation process of a protein A (PA) monolayer. The different pixels of the MS have been properly engineered to monitor different regions of the electromagnetic spectrum. Specifically, these pixels have their plasmonic resonances placed in the range 1500–2000 cm−1, well matched with Amide I and Amide II vibrational bands. In particular, the SEIRA reflectance spectra of the native PA and the denatured PA have been compared, observing a redshift of about 10 cm−1 for both Amide I and Amide II groups. Moreover, the evaluated enhancement factor (up to 7 × 104) allows to reveal both the presence and the denaturation process occurring to a very low amount of PA molecules (about 3 fmoles).
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References
Hu, H., et al.: Recent advances in two-dimensional transition metal dichalcogenides for biological sensing. Biosens. Bioelectron. 142, 111573 (2019)
Wang, J., Lv, F., Liu, L., Ma, Y., Wang, S.: Strategies to design conjugated polymer based materials for biological sensing and imaging. Coord. Chem. Rev. 354, 135–154 (2018)
Lovecchio, N., et al.: Integrated optoelectronic device for detection of fluorescent molecules. IEEE Trans. Biomed. Circuits Syst. 12(6), 1337–1344 (2018)
Kumar, S., Singh, R.: Recent optical sensing technologies for the detection of various biomolecules. Opt. Laser Technol. 134, 106620 (2021)
Stuart, B.H.: Infrared Spectroscopy: Fundamental and Application. Wiley, New York (2004)
Griffiths, P.R.: Fourier Transform Infrared Spectrometry. Wiley, Chichester (2007)
Mayerhöfer, T.G., Pahlow, S., Popp, J.: The Bouguer-Beer-Lambert Law: shining light on the obscure. ChemPhysChem 21(18), 2029–2046 (2020)
Neubrech, F., Huck, C., Weber, K., Pucci, A., Giessen, H.: Surface-enhanced infrared spectroscopy using resonant nanoantennas. Chem. Rev. 117, 5110–5145 (2017)
Vannini, G., Fernàndez-Dominguez, A.I., Heck, S.C., Maier, S.A.: Plasmonic nanoantennas: fundamentals and their use in controlling the radiative properties of nanoemitters. Chem. Rev. 111(6), 3888–3912 (2011)
Di Meo, V., et al.: Metasurface based on cross-shaped plasmonic nanoantennas as chemical sensor for surface-enhanced infrared absorption spectroscopy. Sens. Actuators, B Chem. 286, 600–607 (2019)
Di Meo, V., et al.: Pixeled metasurface for multiwavelength detection of vitamin D. Nanophotonics 9(12), 3921–3930 (2020)
Di Meo, V., et al.: Advanced DNA detection via multispectral plasmonic metasurfaces. Front. Bioeng. Biotechnol. 9, 666121 (2021)
COMSOL Multiphysics homepage. https://www.comsol.it. Accessed 28 Sept 2022
De Marcellis, A., Palange, E., Janneh, M., Rizza, C., Ciattoni, A., Mengali, S.: Design optimisation of plasmonic metasurfaces for mid-infrared high-sensitivity chemical sensing. Plasmonics 12(2), 293–298 (2016). https://doi.org/10.1007/s11468-016-0263-9
Bagheri, S., Weber, K., Gissibl, T., Weiss, T., Neubrech, F., Giessen, H.: Fabrication of square-centimeter plasmonic nanoantenna arrays by femtosecond direct laser writing lithography: effects of collective excitations on SEIRA enhancement. ACS Photonics 2, 779–786 (2015)
Vieu, C., et al.: Electron beam lithography: resolution limits and applications. Appl. Surf. Sci. 164, 111–117 (2000)
Malara, P., et al.: Resonant enhancement of plasmonic nanostructured fiber optic sensors. Sens. Actuators, B Chem. 273, 1587–1592 (2018)
Konermann, L., Pan, J., Liu, Y.H.: Hydrogen exchange mass spectrometry for studying protein structure and dynamics. Chem. Soc. Rev. 40(3), 1224–1234 (2011)
Goormaghtigh, E., Cabiaux, V., Ruysschaert, J.M.: Determination of soluble and membrane protein structure by Fourier transform infrared spectroscopy. II. Experimental aspects, side chain structure, and H/D exchange. Subcell. Biochem. 23, 363–403 (1994)
Lorenz-Fonfria, V.A.: Infrared difference spectroscopy of proteins: from bands to bonds. Chem. Rev. 120(7), 3466–3576 (2020)
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Di Meo, V. et al. (2023). Protein A Denaturation Sensing Through Surface Enhanced InfraRed Absorption (SEIRA) Spectroscopy. In: Cocorullo, G., Crupi, F., Limiti, E. (eds) Proceedings of SIE 2022. SIE 2022. Lecture Notes in Electrical Engineering, vol 1005. Springer, Cham. https://doi.org/10.1007/978-3-031-26066-7_29
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