Abstract
The microsporidium Nosema bombycis can infect and transmit both vertically and horizontally in multiple lepidopteran insects including silkworms and crop pests. While there have been several studies on the N. bombycis spore, there have been only limited studies on the N. bombycis sporoplasm. This chapter reviews what is known about this life cycle stage as well as published studies on purification of the N. bombycis sporoplasm and its survival in an in vitro cell culture system. Genetic transformation techniques have revolutionized the study of many pathogenic organisms. While progress has been made on the development of such systems for microsporidia, this critical problem has not been solved for these pathogens. This chapter provides a summary of the latest research progress on genetic manipulation of N. bombycis.
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This work was supported by grants from the National Natural Science Foundation of China (31,770,159, 31,772,678) and Natural Science Foundation of Chongqing, China (cstc2019yszx-jcyjX0010, cstc2019jcyj-msxmX0511).
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Li, T., Wei, J., Pan, G. (2022). Advances in the Genetic Manipulation of Nosema bombycis. In: Weiss, L.M., Reinke, A.W. (eds) Microsporidia. Experientia Supplementum, vol 114. Springer, Cham. https://doi.org/10.1007/978-3-030-93306-7_6
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